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PMID: 262557 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fluctuations in interbeat interval in rhythmic heart-cell clusters. Role of membrane voltage noise.

Biophysical journal ·Vol. 28 ·No. 3 ·1979-12-00 ·Pages 377-89

Clay JR, DeHaan RL

Abstract

Small clusters of ventricular cells prepared from 7-d chick heart maintain spontaneous, stationary, rhythmic beating in culture for many hours. For clusters containing I-125 cells, mean interbeat interval (IBI) is 0.45 +/- 0.08 s and is independent of cell number (N), whereas, the coefficient of variation of IBI (C) is proportional to N-1/2. Because membrane voltage noise in such clusters would also be expected to vary as N-1/2, we propose a model relating fluctuation in IBI (sigma IBI) to voltage noise (sigma v). A simplified model consisting of random voltage fluctuations superimposed upon a linear pacemaker depolarization of slope a is used to analyze the N-dependent shape of the IBI histogram. Values of sigma v derived from the relation sigma IBI = sigma v/a, or calculated from the skewness of the measured IBI histograms, both agree well with those extrapolated from steady-state noise recorded from resting heart-cell aggregates.

MeSH Terms
Animals Cell Membrane/physiology Cells, Cultured Chick Embryo Electrophysiology Heart/physiology Heart Rate Models, Biological
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clay J R
DeHaan R L
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30 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(79)85187-5
Published
1979-12-00
Pages
377-89
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1328644
Subset
IM
Grants
NHLBI NIH HHS · HL05346 · United States
NHLBI NIH HHS · HL16567 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(79)85187-5 PMC Full Text View journal details
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