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PMID: 26002867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis R-SNARE VAMP721 Interacts with KAT1 and KC1 K+ Channels to Moderate K+ Current at the Plasma Membrane.

The Plant cell ·Vol. 27 ·No. 6 ·2015-06-00 ·Pages 1697-717

Zhang B, Karnik R, Wang Y, Wallmeroth N, Blatt MR, Grefen C

Abstract

SNARE (soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor) proteins drive vesicle traffic, delivering membrane and cargo to target sites within the cell and at its surface. They contribute to cell homeostasis, morphogenesis, and pathogen defense. A subset of SNAREs, including the Arabidopsis thaliana SNARE SYP121, are known also to coordinate solute uptake via physical interactions with K(+) channels and to moderate their gating at the plasma membrane. Here, we identify a second subset of SNAREs that interact to control these K(+) channels, but with opposing actions on gating. We show that VAMPs (vesicle-associated membrane proteins), which target vesicles to the plasma membrane, also interact with and suppress the activities of the inward-rectifying K(+) channels KAT1 and KC1. Interactions were evident in yeast split-ubiquitin assays, they were recovered in vivo by ratiometric bimolecular fluorescence complementation, and they were sensitive to mutation of a single residue, Tyr-57, within the longin domain of VAMP721. Interaction was also recovered on exchange of the residue at this site in the homolog VAMP723, which normally localizes to the endoplasmic reticulum and otherwise did not interact. Functional analysis showed reduced channel activity and alterations in voltage sensitivity that are best explained by a physical interaction with the channel gates. These actions complement those of SYP121, a cognate SNARE partner of VAMP721, and lead us to propose that the channel interactions reflect a "hand-off" in channel control between the two SNARE proteins that is woven together with vesicle fusion.

MeSH Terms
Arabidopsis/metabolism,physiology Arabidopsis Proteins/physiology Cell Membrane/physiology Membrane Potentials/physiology Potassium Channels/physiology Potassium Channels, Inwardly Rectifying/physiology R-SNARE Proteins/physiology Symporters/physiology
Chemicals
Arabidopsis Proteins KAT1 protein, Arabidopsis Potassium Channels Potassium Channels, Inwardly Rectifying R-SNARE Proteins Symporters VAMP721 protein, Arabidopsis potassium-chloride symporters
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhang Ben
Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Karnik Rucha
Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Wang Yizhou
Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Wallmeroth Niklas
ZMBP Developmental Genetics, D-72076 Tuebingen, Germany.
Blatt Michael R
Laboratory of Plant Physiology and Biophysics, University of Glasgow, Glasgow G12 8QQ, United Kingdom michael.blatt@glasgow.ac.uk.
Grefen Christopher
ZMBP Developmental Genetics, D-72076 Tuebingen, Germany.
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.15.00305
Email
michael.blatt@glasgow.ac.uk
Published
2015-06-00
Epub
2015-00-22
Pages
1697-717
Language
English
Region
England
NLM ID
9208688
PMCID
PMC4498211
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/F001630/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/H0024867/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/I024496/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/L001276/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/K015893/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/M01133X/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/H009817/1 · United Kingdom
Corrections
ErratumIn
PMID: 26486448
External Links
PubMed source DOI: 10.1105/tpc.15.00305 PMC Full Text View journal details
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