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- 题目:
- Label-Free Protein-RNA Interactome Analysis Identifies Khsrp Signaling Downstream of the p38/Mk2 Kinase Complex as a Critical Modulator of Cell Cycle Progression.
- 作者:
- Boucas(Jorge),Fritz(Christian),Schmitt(Anna),Riabinska(Arina),Thelen(Lisa),Peifer(Martin),Leeser(Uschi),Nuernberg(Peter),Altmueller(Janine),Gaestel(Matthias),Dieterich(Christoph),Reinhardt(H Christian)
- 状态:
- 发布时间2015-05-21 , 更新时间 2016-11-25
- 期刊:
- PLoS One
- 摘要:
- Growing evidence suggests a key role for RNA binding proteins (RBPs) in genome stability programs. Additionally, recent developments in RNA sequencing technologies, as well as mass-spectrometry techniques, have greatly expanded our knowledge on protein-RNA interactions. We here use full transcriptome sequencing and label-free LC/MS/MS to identify global changes in protein-RNA interactions in response to etoposide-induced genotoxic stress. We show that RBPs have distinct binding patterns in response to genotoxic stress and that inactivation of the RBP regulator module, p38/MK2, can affect the entire spectrum of protein-RNA interactions that take place in response to stress. In addition to validating the role of known RBPs like Srsf1, Srsf2, Elavl1 in the genotoxic stress response, we add a new collection of RBPs to the DNA damage response. We identify Khsrp as a highly regulated RBP in response to genotoxic stress and further validate its role as a driver of the G(1/)S transition through the suppression of Cdkn1a(P21) transcripts. Finally, we identify KHSRP as an indicator of overall survival, as well as disease free survival in glioblastoma multiforme.
- 语言:
- eng
- DOI:
DataTable pData
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