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PMID: 2582354 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The gene for an exported antigen of the malaria parasite Plasmodium falciparum cloned and expressed in Escherichia coli.

Nucleic acids research ·Vol. 13 ·No. 2 ·1985-01-25 ·Pages 369-79

Hope IA, Mackay M, Hyde JE, Goman M, Scaife J

Abstract

An exported protein of the erythrocytic stages of the malaria parasite, Plasmodium falciparum, has epitope(s) in common with the surface of the sporozoite stage (1). Two cDNA clones encoding this protein, Ag5.1, have now been isolated and expressed in Escherichia coli. The coding sequence contains a region with strong homology to that of the circumsporozoite protein of P. falciparum. Other features of the sequence can be explained in terms of the observed behaviour of the protein in the parasite life cycle. The Ag5.1 can now be synthesised in bacteria in sufficient amounts to analyse the immune response to this protein.

MeSH Terms
Amino Acid Sequence Antibody Specificity Antigens, Protozoan/genetics Base Sequence Cloning, Molecular DNA/genetics Epitopes Erythrocytes/parasitology Escherichia coli/genetics Gene Expression Regulation Molecular Weight Peptides/genetics Plasmodium falciparum/genetics,immunology Protein Sorting Signals RNA, Messenger/genetics
Chemicals
Antigens, Protozoan Epitopes Peptides Protein Sorting Signals RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hope I A
Mackay M
Hyde J E
Goman M
Scaife J
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.2.369
Published
1985-01-25
Pages
369-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341001
Subset
IM
Databases
GENBANK
X01745
External Links
PubMed source DOI: 10.1093/nar/13.2.369 PMC Full Text View journal details
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