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PMID: 2580443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cellular differentiation of epithelioid sarcoma. An electron-microscopic, enzyme-histochemical, and immunohistochemical study.

The American journal of pathology ·Vol. 119 ·No. 1 ·1985-04-00 ·Pages 44-56

Mukai M, Torikata C, Iri H, Hanaoka H, Kawai T, Yakumaru K, Shimoda T, Mikata A, Kageyama K

Abstract

For the purpose of clarifying cellular differentiation of epithelioid sarcoma, studies based on various methods were performed. Enzyme histochemical studies showed that epithelioid sarcoma tumor cells have characteristics intermediate between epithelial cells and the large plump cells of synovial sarcoma-incomplete epithelial differentiation. For alkaline phosphatase and adenosine triphosphatase particularly, positive cells and negative cells coexisted, as in the large plump cells of synovial sarcoma. Immunohistochemical studies for alpha 1-antitrypsin, alpha 1-antichymotrypsin, vimentin, and keratin also showed that epithelioid sarcoma tumor cells are very similar to the large plump cells of synovial sarcoma and have incomplete epithelial differentiation. For example, the examinations of serial sections and double staining methods revealed that keratin-positive cells are always vimentin-positive in epithelioid sarcoma and in the monophasic area of synovial sarcoma. Electron-microscopically, bundles of intermediate filaments and filopodia toward the intercellular lumen were observed, as in the monophasic area of synovial sarcoma. The results of enzyme-histochemical and immunohistochemical studies of non-neoplastic synovial lining cells, performed here for the first time, are also discussed.

MeSH Terms
Adenosine Triphosphatases/metabolism Adult Alkaline Phosphatase/metabolism Cell Transformation, Neoplastic/pathology Female Fibroma/pathology,ultrastructure Humans Keratins/metabolism Male Microscopy, Electron Middle Aged Sarcoma/pathology,ultrastructure Sarcoma, Synovial/pathology,ultrastructure Staining and Labeling Vimentin/metabolism
Chemicals
Vimentin Keratins Alkaline Phosphatase Adenosine Triphosphatases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Mukai M
Torikata C
Iri H
Hanaoka H
Kawai T
Yakumaru K
Shimoda T
Mikata A
Kageyama K
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1985-04-00
Pages
44-56
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1888074
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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