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PMID: 2579956 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proteins transported in slow components a and b of axonal transport are distributed differently in the transverse plane of the axon.

The Journal of cell biology ·Vol. 100 ·No. 4 ·1985-04-00 ·Pages 1167-72

Heriot K, Gambetti P, Lasek RJ

Abstract

The distribution of the proteins migrating with the slow components a (SCa) and b (SCb) of axonal transport were studied in cross-sections of axons with electron microscope autoradiography. Radiolabeled amino acids were injected into the hypoglossal nucleus of rabbits and after 15 d, the animals were killed. Hypoglossal nerves were processed either for SDS-polyacrylamide gel electrophoresis fluorography to identify and locate the two components of slow transport, or for quantitative electron microscope autoradiography. Proteins transported in SCa were found to be uniformly distributed within the cross-section of the axon. Labeled SCb proteins were also found throughout the axonal cross-section, but the subaxolemmal region of the axon contained 2.5 times more SCb radioactivity than any comparable area in the remainder of the axon.

MeSH Terms
Actins/metabolism Animals Autoradiography Axonal Transport Hypoglossal Nerve/metabolism Intermediate Filament Proteins/metabolism Kinetics Microscopy, Electron Microtubule Proteins/metabolism Nerve Tissue Proteins/metabolism Rabbits Tubulin/metabolism
Chemicals
Actins Intermediate Filament Proteins Microtubule Proteins Nerve Tissue Proteins Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heriot K
Gambetti P
Lasek R J
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33 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.100.4.1167
Published
1985-04-00
Pages
1167-72
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113744
Subset
IM
Grants
NIA NIH HHS · AG00795 · United States
NINDS NIH HHS · NS14509 · United States
NINDS NIH HHS · NS15731 · United States
External Links
PubMed source DOI: 10.1083/jcb.100.4.1167 PMC Full Text View journal details
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