Home LiteratureArticle Details
PMID: 2574822 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of intron splicing in the function of the MATa1 gene of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4613-20

Ner SS, Smith M

Abstract

The MATa1 gene of Saccharomyces cerevisiae is unique in yeast cells in that it contains two short intervening sequences (IVS1 and IVS2) 54 and 51 nucleotides long. The 3' intron is inefficiently spliced and results in the accumulation of transcript with only the first intron removed, leading to the speculation that the gene may produce different protein products by alternative splicing patterns. We have used in vitro mutagenic techniques to construct intronless MATa1 genes and have introduced point substitutions in the 5'-TACTAAC-3' internal conserved sequence of each intron to identify the protein product that is required for repression of haploid-specific genes. Analysis of these constructs for the ability to repress expression of an HO::lacZ fusion and for the ability to allow diploid cells to undergo sporulation during conditions of starvation revealed that the gene is functional with two, one, or no introns and that the only functional protein is the one produced when both introns are spliced from the mRNA.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes, Fungal Genes, Homeobox Haploidy Introns Lac Operon Molecular Sequence Data Mutation Plasmids RNA Splicing Restriction Mapping Saccharomyces cerevisiae/genetics,physiology Spores, Fungal Transcription, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ner S S
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
Smith M
References (32)
32 references, click to expand
  1. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.
    J Cell Biol. 1980 Jun;85(3):811-22 PMID: 6993497
  2. A rapid droplet method for Sanger dideoxy sequencing.
    Biotechniques. 1988 May;6(5):408, 410, 412 PMID: 3273403
  3. Physical analysis of mating-type loci in Saccharomyces cerevisiae.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:961-81 PMID: 7021055
  4. Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis.
    J Mol Biol. 1981 Apr 15;147(3):357-72 PMID: 7031257
  5. The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae.
    Cell. 1981 Nov;27(1 Pt 2):15-23 PMID: 7034964
  6. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing.
    Cell. 1983 Sep;34(2):395-403 PMID: 6616616
  7. Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.
    Cell. 1984 Mar;36(3):645-53 PMID: 6365330
  8. The yeast MATa1 gene contains two introns.
    EMBO J. 1984 May;3(5):1061-5 PMID: 6329735
  9. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  10. Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins.
    Nature. 1984 Jul 5-11;310(5972):70-1 PMID: 6429549
  11. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
    Cell. 1984 Aug;38(1):317-31 PMID: 6088074
  12. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.
    Cell. 1984 Dec;39(3 Pt 2):611-21 PMID: 6096014
  13. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  14. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  15. A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligation.
    Cell. 1985 May;41(1):107-18 PMID: 2986840
  16. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  17. The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction.
    Science. 1985 May 24;228(4702):963-7 PMID: 3890181
  18. Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro.
    Cell. 1985 Aug;42(1):335-44 PMID: 3893746
  19. A multicomponent complex is involved in the splicing of messenger RNA precursors.
    Cell. 1985 Aug;42(1):345-53 PMID: 3160482
  20. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
    Cell. 1985 Aug;42(1):355-67 PMID: 3160483
  21. A quantitative analysis of the effects of 5' junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing.
    Cell. 1985 Dec;43(2 Pt 1):423-30 PMID: 3935321
  22. Activation of meiosis and sporulation by repression of the RME1 product in yeast.
    Nature. 1986 Feb 27-Mar 5;319(6056):738-42 PMID: 3513021
  23. Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing.
    Cell. 1986 Apr 11;45(1):81-93 PMID: 3513966
  24. Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs.
    Mol Cell Biol. 1985 Dec;5(12):3545-51 PMID: 3915780
  25. Structure of the Saccharomyces cerevisiae HO gene and analysis of its upstream regulatory region.
    Mol Cell Biol. 1986 Dec;6(12):4281-94 PMID: 3025649
  26. Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.
    Mol Cell Biol. 1987 Jan;7(1):225-30 PMID: 3550418
  27. Spliceosome assembly in yeast.
    Genes Dev. 1987 Nov;1(9):1014-27 PMID: 2962902
  28. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  29. a1 protein alters the DNA binding specificity of alpha 2 repressor.
    Cell. 1988 Mar 25;52(6):875-82 PMID: 3127056
  30. A simple and efficient procedure for generating random point mutations and for codon replacements using mixed oligodeoxynucleotides.
    DNA. 1988 Mar;7(2):127-34 PMID: 3282853
  31. Life cycle of the budding yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Dec;52(4):536-53 PMID: 3070323
  32. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4613-4620.1989
Published
1989-11-00
Pages
4613-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363607
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.11.4613-4620.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2