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PMID: 25700270 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

NME2 reduces proliferation, migration and invasion of gastric cancer cells to limit metastasis.

PloS one ·Vol. 10 ·No. 2 ·2015-00-00 ·Pages e0115968

Liu YF, Yang A, Liu W, Wang C, Wang M, Zhang L, Wang D, Dong JF, Li M

Abstract

Gastric cancer is one of the most common malignancies and has a high rate of metastasis. We hypothesize that NME2 (Nucleoside Diphosphate Kinase 2), which has previously been considered as an anti-metastatic gene, plays a role in the invasiveness of gastric cancer cells. Using a tissue chip technology and immunohistochemistry, we demonstrated that NME2 expression was associated with levels of differentiation of gastric cancer cells and their metastasis into the lymph nodes. When the NME2 gene product was over-expressed by ;in vitro stable transfection, cells from BGC823 and MKN45 gastric cancer cell lines had reduced rates of proliferation, migration, and invasion through the collagen matrix, suggesting an inhibitory activity of NME2 in the propagation and invasion of gastric cancer. NME2 could, therefore, severe as a risk marker for gastric cancer invasiveness and a potential new target for gene therapy to enhance or induce NME2 expression.

MeSH Terms
Adenocarcinoma/enzymology,secondary Cell Line, Tumor Cell Movement Cell Proliferation Female Humans Male Middle Aged NM23 Nucleoside Diphosphate Kinases/physiology Neoplasm Invasiveness Stomach Neoplasms/enzymology,pathology
Chemicals
NM23 Nucleoside Diphosphate Kinases NME2 protein, human
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Liu Yan-fei
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Department of Pathology, Xi'an Children's Hospital, Xi'an, China.
Yang Aijun
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Liu Wei
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Wang Chenyu
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Wang Min
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Zhang Lihan
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Wang Dongcang
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
Dong Jing-fei
Puget Sound Blood Center, Seattle, Washington, United States of America; Division of Hematology, Department of Medicine, University of Washington, School of Medicine, Seattle, Washington, United States of America.
Li Min
Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, Lanzhou, China.
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0115968
Published
2015-00-00
Epub
2015-00-20
Pages
e0115968
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC4336288
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0115968 PMC Full Text View journal details
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