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PMID: 2564416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human lymphocytes bearing T cell receptor gamma/delta are phenotypically diverse and evenly distributed throughout the lymphoid system.

The Journal of experimental medicine ·Vol. 169 ·No. 4 ·1989-04-01 ·Pages 1277-94

Groh V, Porcelli S, Fabbi M, Lanier LL, Picker LJ, Anderson T, Warnke RA, Bhan AK, Strominger JL, Brenner MB

Abstract

A direct quantitative and phenotypic cytofluorographic analysis of TCR-gamma/delta+ lymphocytes as well as an immunohistologic study of their tissue distribution and microanatomy was made possible by the availability of two mAbs (anti-TCR-delta 1 and anti-C gamma M1) specific for framework determinants on human TCR gamma and delta chains, respectively. TCR-gamma/delta+ lymphocytes, ranging between greater than 0.5 and 16% of CD3+ cells, were found in fetal and postnatal thymus, fetal and adult peripheral lymphoid organs, and adult peripheral blood. While TCR-gamma/delta+ lymphocytes comprised a small subpopulation of T cells (mean, approximately 4%) occasionally greater than 10-16% of CD3+ cells expressed TCR-gamma/delta. Virtually all TCR-gamma/delta+ thymocytes/lymphocytes expressed CD7, CD2, and CD5 but were heterogeneous with respect to their expression of CD1, CD4, CD8, CD28, CD11b, CD16, and Leu-7. Human TCR-gamma/delta+ cells populate both organized lymphoid tissues (thymus, tonsil, lymphnode, and spleen) as well as the gut- and skin-associated lymphoid systems at similar frequencies without obvious tropism for epithelial microenvironments. TCR-gamma/delta+ lymphocytes tend to be located within a given organ wherever TCR-alpha/beta+ lymphocytes are found. This study shows that TCR-gamma/delta+ lymphocytes constitute a small but numerically important, phenotypically diverse T cell population distributed throughout the body. These results support the concept that TCR-gamma/delta+ cells comprise a distinct, functionally heterogeneous, mature T cell sublineage that may substantially broaden the T cell repertoire at all immunologically relevant sites.

MeSH Terms
Antibodies, Monoclonal/immunology CD4-Positive T-Lymphocytes/immunology Flow Cytometry Humans Immunoenzyme Techniques Leukocyte Count Lymphoid Tissue/cytology Receptors, Antigen, T-Cell/physiology Receptors, Antigen, T-Cell, gamma-delta T-Lymphocytes/classification,immunology Tissue Distribution
Chemicals
Antibodies, Monoclonal Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, gamma-delta
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Groh V
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Porcelli S
Fabbi M
Lanier L L
Picker L J
Anderson T
Warnke R A
Bhan A K
Strominger J L
Brenner M B
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.169.4.1277
Published
1989-04-01
Pages
1277-94
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189233
Subset
IM
Grants
NIAID NIH HHS · AI-10736 · United States
NIAID NIH HHS · AI-15669 · United States
NHLBI NIH HHS · HL-18646 · United States
External Links
PubMed source DOI: 10.1084/jem.169.4.1277 PMC Full Text View journal details
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