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PMID: 2563367 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation-deficient mutants of piliated Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 171 ·No. 2 ·1989-02-00 ·Pages 657-64

Biswas GD, Lacks SA, Sparling PF

Abstract

Seven transformation-deficient mutants of piliated, competent Neisseria gonorrhoeae were isolated by screening them for their inability to be transformed by chromosomal DNA after chemical mutagenesis. Three distinct classes of mutants were obtained, each of which was piliated, as determined by electron microscopy. One class exhibited abnormal colony morphology and was unable to take up DNA into a DNase-resistant state. A second class was morphologically normal and took up DNA into a DNase-resistant state normally, but was deficient in both chromosomal and plasmid transformation; mutations in these mutants may affect entry of DNA into the cell proper. A third class was similar to the second but was fully competent for plasmid transformation, suggesting that there was a defect in a late stage of chromosomal transformation.

MeSH Terms
Chromosomes, Bacterial DNA, Bacterial/genetics Deoxyribonucleases/metabolism Fimbriae, Bacterial/ultrastructure Microscopy, Electron Mutation Neisseria gonorrhoeae/genetics,radiation effects,ultrastructure Plasmids Transformation, Bacterial Ultraviolet Rays
Chemicals
DNA, Bacterial Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Biswas G D
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.
Lacks S A
Sparling P F
References (33)
33 references, click to expand
  1. Nuclease detection in SDS-polyacrylamide gel electrophoresis.
    Anal Biochem. 1977 May 15;80(1):76-90 PMID: 18945
  2. Multiple protein differences exist between Neisseria gonorrhoeae type 1 and type 4.
    Infect Immun. 1988 Apr;56(4):808-14 PMID: 3126144
  3. Specificity of DNA uptake in genetic transformation of gonococci.
    Biochem Biophys Res Commun. 1979 Jan 15;86(1):97-104 PMID: 107953
  4. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
    J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
  5. Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function.
    J Bacteriol. 1979 May;138(2):510-8 PMID: 108261
  6. Marker rescue transformation by linear plasmid DNA in Bacillus subtilis.
    Plasmid. 1979 Oct;2(4):555-71 PMID: 119244
  7. Entry of double-stranded deoxyribonucleic acid during transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1981 Jan;145(1):638-40 PMID: 6780524
  8. Plasmid transformation in Haemophilus influenzae.
    J Bacteriol. 1981 Feb;145(2):1099-101 PMID: 6970194
  9. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  10. Marker rescue by a homologous recipient plasmid during transformation of gonococci by a hybrid Pcr plasmid.
    J Bacteriol. 1982 Jul;151(1):77-82 PMID: 6282813
  11. Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1982 Dec;152(3):1071-7 PMID: 6292160
  12. Possible mechanism for donor DNA binding and transport in Haemophilus.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6370-4 PMID: 6959125
  13. Directional transport and integration of donor DNA in Haemophilus influenzae transformation.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7274-8 PMID: 6316352
  14. The genetics of the gonococcus.
    Annu Rev Microbiol. 1984;38:111-33 PMID: 6437322
  15. Role of chromosomal rearrangement in N. gonorrhoeae pilus phase variation.
    Cell. 1985 Feb;40(2):293-300 PMID: 2857113
  16. On the role of pili in transformation of Neisseria gonorrhoeae.
    J Gen Microbiol. 1984 Dec;130(12):3165-73 PMID: 6151587
  17. Recombination near the antibiotic resistance locus penB results in antigenic variation of gonococcal outer membrane protein I.
    Infect Immun. 1986 May;52(2):529-33 PMID: 2422125
  18. Piliation control mechanisms in Neisseria gonorrhoeae.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3890-4 PMID: 2872674
  19. Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid.
    J Bacteriol. 1986 Aug;167(2):685-94 PMID: 3090021
  20. Linearization of donor DNA during plasmid transformation in Neisseria gonorrhoeae.
    J Bacteriol. 1986 Nov;168(2):756-61 PMID: 3096959
  21. Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants.
    J Bacteriol. 1987 Feb;169(2):790-5 PMID: 3100504
  22. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance.
    J Bacteriol. 1966 Nov;92(5):1364-71 PMID: 4958881
  23. Colonial morphology of Neisseria gonorrhoeae isolated from males and females.
    J Bacteriol. 1967 Jan;93(1):513 PMID: 4960158
  24. Competence mutants: isolation of transformation deficient strains of Haemophilus influenzae.
    Nature. 1970 Aug 1;227(5257):515-7 PMID: 5310646
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):437-9 PMID: 4997136
  27. Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns.
    J Exp Med. 1971 Oct 1;134(4):886-906 PMID: 4106489
  28. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing.
    J Infect Dis. 1973 Aug;128(2):178-94 PMID: 4198720
  29. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  30. Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2305-9 PMID: 4152205
  31. Factors affecting genetic transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1977 Feb;129(2):983-92 PMID: 14116
  32. Altered outer membrane protein in different colonial types of Neisseria gonorrhoeae.
    J Bacteriol. 1977 Mar;129(3):1623-7 PMID: 403181
  33. Preferential uptake of restriction fragments from a gonococcal cryptic plasmid by competent Neisseria gonorrhoeae.
    J Gen Microbiol. 1988 Mar;134(3):547-57 PMID: 3141569
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.2.657-664.1989
Published
1989-02-00
Pages
657-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209647
Subset
IM
Grants
NIAID NIH HHS · AI14885 · United States
NIAID NIH HHS · AI15036 · United States
External Links
PubMed source DOI: 10.1128/jb.171.2.657-664.1989 PMC Full Text View journal details
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