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PMID: 2557843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphatidylinositol 4,5-bisphosphate hydrolysis in human sperm stimulated with follicular fluid or progesterone is dependent upon Ca2+ influx.

The Biochemical journal ·Vol. 264 ·No. 2 ·1989-12-01 ·Pages 539-46

Thomas P, Meizel S

Abstract

Hydrolysis of the phospholipid phosphatidylinositol 4,5-bisphosphate is thought to be intimately involved in agonist-induced changes in intracellular Ca2+ levels. Recently we have shown that human preovulatory follicular fluid, which induces exocytosis in human sperm, can stimulate a rapid, transient increase in sperm cytosolic [Ca2+] [Thomas & Meizel (1988) Gamete Res. 20, 397-411]. We report here that both a Sephadex G-75 column fraction, derived from follicular fluid, and progesterone (a component of both the G-75 fraction and whole follicular fluid) stimulate rapid hydrolysis of PtdIns(4,5)P2 and PtdIns4P in human sperm. We also report that progesterone stimulates a rapid influx of Ca2+ in human sperm. Human spermatozoa were labelled for 24 h with myo-[3H]inositol and then treated with either the G-75 fraction or progesterone. A 30-65% loss of label was detected in PtdIns(4,5)P2 and PtdIns4P within 15 s of stimulus addition; no changes were observed in PtdIns during 2 min of treatment. The loss of label from both lipids was accompanied by an increase in water-soluble inositol phosphates. Production of both InsP3 and InsP2 was seen within 10 s; however, InsP3 was rapidly removed and had reached control levels by 1 min. Similarly, formation of InsP2 reached a peak by 30 s and then began a decline accompanied by a corresponding increase in InsP. No increases in InsP4 were seen in sperm treated in this fashion. Stimulated hydrolysis of the phosphoinositides and release of inositol phosphates were both blocked by the Ca2+ antagonist La3+. Likewise, the progesterone-induced increase in intracellular Ca2+ was inhibited by La3+, and phosphoinositide hydrolysis stimulated by this hormone was dependent upon the presence of extracellular Ca2+.

MeSH Terms
Calcium/metabolism Chromatography, High Pressure Liquid Female Humans Hydrolysis In Vitro Techniques Inositol/metabolism Inositol Phosphates/isolation & purification,metabolism Kinetics Male Ovarian Follicle/physiology Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols/isolation & purification,metabolism Phytic Acid/metabolism Progesterone/pharmacology Spermatozoa/drug effects,metabolism
Chemicals
Inositol Phosphates Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Progesterone Inositol Phytic Acid Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thomas P
Department of Human Anatomy, School of Medicine, University of California, Davis 95616.
Meizel S
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2640539
Published
1989-12-01
Pages
539-46
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133613
Subset
IM
Grants
NICHD NIH HHS · HD06698 · United States
External Links
PubMed source DOI: 10.1042/bj2640539 PMC Full Text View journal details
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