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PMID: 2554859 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120.

Archives of virology ·Vol. 107 ·No. 3-4 ·1989-00-00 ·Pages 301-5

Hemming A, Lundberg L, Olofsson S

Abstract

By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for further mutagenesis and is also easy to reclone.

MeSH Terms

Amino Acid Sequence Bacteriophages/genetics Binding Sites Cloning, Molecular Codon Genetic Vectors HIV Envelope Protein gp120/genetics,metabolism Mutation Oligonucleotides/chemical synthesis Receptors, HIV/metabolism

Chemicals

Codon HIV Envelope Protein gp120 Oligonucleotides Receptors, HIV

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hemming A

Department of Clinical Virology, University of Göteborg, Sweden.

Lundberg L

Olofsson S

References (8)

8 references, click to expand

Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis.
Nucleic Acids Res. 1986 Dec 22;14(24):9679-98 PMID: 3027659
Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.
Science. 1987 Sep 11;237(4820):1351-5 PMID: 3629244
Coexpression of human immunodeficiency virus envelope proteins and tat from a single simian virus 40 late replacement vector.
Proc Natl Acad Sci U S A. 1988 Jan;85(2):334-8 PMID: 2829181
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor.
Cell. 1987 Sep 11;50(6):975-85 PMID: 2441877
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
Improved oligonucleotide site-directed mutagenesis using M13 vectors.
Nucleic Acids Res. 1985 Jun 25;13(12):4431-43 PMID: 2989795
95- and 25-kDa fragments of the human immunodeficiency virus envelope glycoprotein gp120 bind to the CD4 receptor.
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6543-6 PMID: 2842778

Article Info

Journal

Archives of virology

Abbr.

Arch Virol

ISSN

0304-8608

DOI

10.1007/BF01317925

Published

1989-00-00

Pages

301-5

Language

English

Region

Austria

NLM ID

7506870

Subset

External Links

PubMed source DOI: 10.1007/BF01317925 View journal details

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A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120.

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