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PMID: 2553769 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Serological characterization of bovine rotaviruses isolated from dairy and beef herds in Argentina.

Journal of clinical microbiology ·Vol. 27 ·No. 11 ·1989-11-00 ·Pages 2619-23

Bellinzoni RC, Blackhall JO, Mattion NM, Estes MK, Snodgrass DR, LaTorre JL, Scodeller EA

Abstract

Bovine rotaviruses isolated from beef and dairy herds in Argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (G. Gerna, M. Battaglia, G. Milenesi, N. Passarani, E. Percivalle, and E. Cattaneo, Infect. Immun. 43:722-729, 1984). Three strains from beef herds were related to the UK and NCDV bovine rotavirus strains defined as serotype 6 (Y. Hoshino, R. G. Wyatt, H. B. Greenberg, J. Flores, and A. Z. Kapikian, J. Infect. Dis. 149:694-702, 1984). Two other strains from dairy herds were classified as bovine viruses related to the bovine B223 strain reported by Woode and co-workers (G. N. Woode, N. E. Kelso, T. F. Simpson, S. K. Gaul, L. E. Evans, and L. Babiuk, J. Clin. Microbiol. 18:358-364, 1983) in the United States. A serotyping antibody-capture enzyme-linked immunoassay to detect serotype 6 rotavirus using a serotype 6-specific monoclonal antibody was developed and evaluated for strain characterization. Characterization of 72 group A rotavirus-positive fecal samples from beef herds and 43 fecal samples from dairy herds showed a predominance of serotype 6 rotavirus in beef herds but both serotype 6 and non-serotype 6 rotaviruses in dairy herds. Analysis of genomic double-stranded RNA by polyacrylamide gel electrophoresis showed that when outbreaks were caused by one serotype only a single electropherotype was present in all samples.

MeSH Terms
Animals Antigenic Variation Argentina Cattle Cattle Diseases/microbiology Cross Reactions Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Immunoenzyme Techniques Neutralization Tests RNA, Viral/analysis Rotavirus/classification,genetics,immunology Rotavirus Infections/microbiology,veterinary Serotyping
Chemicals
RNA, Viral
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bellinzoni R C
Centro de Virologia Animal, Argentina.
Blackhall J O
Mattion N M
Estes M K
Snodgrass D R
LaTorre J L
Scodeller E A
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.27.11.2619-2623.1989
Published
1989-11-00
Pages
2619-23
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267089
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.27.11.2619-2623.1989 PMC Full Text View journal details
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