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PMID: 2552182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation.

Journal of virology ·Vol. 63 ·No. 11 ·1989-11-00 ·Pages 4986-90

Hensel CH, Petersen RB, Hackett PB

Abstract

The 372-nucleotide leader sequence of Rous sarcoma virus RNA contains three conserved short open reading frames and other sequences responsible for a variety of life cycle functions. We have investigated several aspects of the leader RNA which may influence the translation of the major coding regions to which the leader is juxtaposed. We found that small perturbations of the leader length do not affect the binding and scanning of ribosomal subunits by more than about 10%, that the length and/or structure of the RSV RNA leader is near optimal for translation of the major coding regions of the viral RNA, that inclusion or deletion of open reading frames influences downstream initiation in a manner that is not strictly additive, and that reinitiation of translation at the gag gene is very efficient.

MeSH Terms
Avian Sarcoma Viruses/genetics Base Sequence Cloning, Molecular Gene Products, gag/genetics Genes, Viral Molecular Sequence Data Mutation Peptide Chain Initiation, Translational Plasmids Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Ribosomes/metabolism Viral Structural Proteins/genetics
Chemicals
Gene Products, gag RNA, Messenger RNA, Viral Viral Structural Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hensel C H
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
Petersen R B
Hackett P B
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.11.4986-4990.1989
Published
1989-11-00
Pages
4986-90
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC251153
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.11.4986-4990.1989 PMC Full Text View journal details
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