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PMID: 2542604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Modulation of arachidonic acid metabolism by Rous sarcoma virus.

Journal of virology ·Vol. 63 ·No. 7 ·1989-07-00 ·Pages 2929-35

Barker K, Aderem A, Hanafusa H

Abstract

Arachidonic acid (C20:4) metabolites were released constitutively from wild-type Rous sarcoma virus-transformed chicken embryo fibroblasts (CEF). 3H-labeled C20:4 and its metabolites were released from unstimulated and uninfected CEF only in response to stimuli such as serum, phorbol ester, or the calcium ionophore A23187. High-pressure liquid chromatography analysis showed that the radioactivity released from [3H]arachidonate-labeled transformed cells was contained in free arachidonate and in the cyclooxygenase products prostaglandin E2 and prostaglandin F2 alpha; no lipoxygenase products were identified. The release of C20:4 and its metabolites from CEF infected with pp60src deletion mutants was correlated with serum-independent DNA synthesis and with the expression of the mRNA for 9E3, a gene expressed in Rous sarcoma virus-transformed cells which has homology with several mitogenic and inflammatory peptides. 3H-labeled C20:4 release was not correlated with p36 phosphorylation, which argues against a role for this protein as a phospholipase A2 inhibitor. CEF infected with other oncogenic viruses encoding a tyrosine kinase also released C20:4, as did CEF infected with viruses that contained mos and ras; however, infection with a crk-containing virus did not result in stimulation of 3H-labeled C20:4 release, suggesting that utilization of this signaling pathway is specific for particular transformation stimuli.

MeSH Terms
Animals Arachidonic Acids/metabolism Avian Sarcoma Viruses/genetics Blotting, Northern Calcimycin/pharmacology Cell Transformation, Neoplastic Cells, Cultured Chick Embryo DNA Replication Nucleic Acid Hybridization RNA, Messenger/genetics Tritium
Chemicals
Arachidonic Acids RNA, Messenger Tritium Calcimycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barker K
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021-6399.
Aderem A
Hanafusa H
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48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.7.2929-2935.1989
Published
1989-07-00
Pages
2929-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250846
Subset
IM
Grants
NIAID NIH HHS · AI07233 · United States
NCI NIH HHS · CA44356 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.7.2929-2935.1989 PMC Full Text View journal details
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