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PMID: 2539353 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Establishment of gene transfer systems for and construction of the genetic map of a marine Vibrio strain.

Journal of bacteriology ·Vol. 171 ·No. 4 ·1989-04-00 ·Pages 1825-34

Ichige A, Matsutani S, Oishi K, Mizushima S

Abstract

Two gene transfer systems were established for a marine bacterium, Vibrio sp. strain 60. One was generalized transduction with a newly isolated bacteriophage, As3, and the other was conjugal gene transfer by the use of newly constructed transposon-facilitated recombination (Tfr) donors. As3 transduced various chromosomal markers at frequencies of 10(-4) to 10(-6). Tfr donors, which were constructed by introducing transposon Tn10 into both plasmid RP4 and the chromosome, mediated the polarized transfer of chromosomal genes from the sites of Tn10 insertion on the chromosome. By means of these gene transfer systems, a genetic map of the vibrio chromosome was constructed.

MeSH Terms
Bacteriophages/genetics Chromosome Mapping DNA Transposable Elements DNA, Bacterial/genetics Genes, Bacterial Genetic Linkage Selection, Genetic Transduction, Genetic Vibrio/genetics
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ichige A
Institute of Applied Microbiology, University of Tokyo, Japan.
Matsutani S
Oishi K
Mizushima S
References (27)
27 references, click to expand
  1. Mapping of chromosomal genes that determine the El Tor biotype in Vibrio cholerae.
    Infect Immun. 1983 Dec;42(3):924-9 PMID: 6315594
  2. Transposon-facilitated recombination in Vibrio cholerae.
    Mol Gen Genet. 1979 Feb 16;170(1):93-101 PMID: 286148
  3. Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species.
    J Bacteriol. 1970 Oct;104(1):434-42 PMID: 5473902
  4. Properties of an R factor from Pseudomonas aeruginosa.
    J Bacteriol. 1971 Dec;108(3):1244-9 PMID: 4945193
  5. Transposon insertion mutagenesis of Pseudomonas aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster.
    Gene. 1985;33(3):293-303 PMID: 2989092
  6. N2-fixing vibrios isolated from the gastrointestinal tract of sea urchins.
    Can J Microbiol. 1981 Mar;27(3):311-7 PMID: 6940646
  7. Numerical taxonomy of phenanthrene-degrading bacteria isolated from the Chesapeake Bay.
    Appl Environ Microbiol. 1984 Nov;48(5):988-93 PMID: 6508314
  8. Transposon-mediated mutagenesis and recombination in Vibrio cholerae.
    Infect Immun. 1984 Aug;45(2):428-32 PMID: 6086528
  9. Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation.
    Annu Rev Microbiol. 1987;41:77-101 PMID: 3318684
  10. Transduction by bacteriophage T1.
    Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083-8 PMID: 4920089
  11. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  12. Expanded linkage map of Vibrio cholerae.
    Genetics. 1979 Feb;91(2):191-214 PMID: 447068
  13. Bacterial flora of phyto- and zoo-plankton in the inshore water of Japan.
    Can J Microbiol. 1971 Sep;17(9):1157-60 PMID: 4939918
  14. Transposon-facilitated recombination in classical biotypes of Vibrio cholerae.
    Infect Immun. 1981 Jun;32(3):1132-8 PMID: 6265372
  15. Plasmids that mobilize bacterial chromosome.
    Plasmid. 1979 Jan;2(1):1-19 PMID: 382197
  16. Genetics of Vibrio cholerae and its bacteriophages.
    Microbiol Rev. 1987 Jun;51(2):285-98 PMID: 3299030
  17. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.
    Mol Gen Genet. 1976 Mar 30;144(3):243-51 PMID: 818504
  18. Diseases of humans (other than cholera) caused by vibrios.
    Annu Rev Microbiol. 1980;34:341-67 PMID: 7002028
  19. Isolation of spontaneously derived mutants of Caulobacter crescentus.
    Genetics. 1977 May;86(1):25-32 PMID: 407126
  20. Isolation and characterization of mutants of a marine Vibrio strain that are defective in production of extracellular proteins.
    J Bacteriol. 1988 Aug;170(8):3537-42 PMID: 2457013
  21. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  22. Peptone induction and rifampin-insensitive collagenase production by Vibrio alginolyticus.
    J Bacteriol. 1980 May;142(2):447-54 PMID: 6247322
  23. Exohemagglutinins: new products of vibrios.
    Appl Environ Microbiol. 1979 Jul;38(1):169-72 PMID: 485152
  24. Transduction in Bacillus subtilis by bacteriophage SPP1.
    J Virol. 1974 Dec;14(6):1343-8 PMID: 4214946
  25. Generalized Transduction in CAULOBACTER CRESCENTUS.
    Genetics. 1977 Nov;87(3):391-9 PMID: 17248770
  26. Regulation of extracellular alkaline protease activity by histidine in a collagenolytic Vibrio alginolyticus strain.
    J Gen Microbiol. 1981 Nov;127(1):193-9 PMID: 6279766
  27. General Transduction in Vibrio cholerae.
    Infect Immun. 1981 Feb;31(2):737-41 PMID: 7216473
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.4.1825-1834.1989
Published
1989-04-00
Pages
1825-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209828
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.4.1825-1834.1989 PMC Full Text View journal details
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