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PMID: 2537838 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Type I collagen gel induces Madin-Darby canine kidney cells to become fusiform in shape and lose apical-basal polarity.

The Journal of cell biology ·Vol. 108 ·No. 3 ·1989-03-00 ·Pages 903-19

Zuk A, Matlin KS, Hay ED

Abstract

In the embryo, epithelia give rise to mesenchyme at specific times and places. Recently, it has been reported (Greenburg, G., and E. D. Hay. 1986. Dev. Biol. 115:363-379; Greenberg, G., and E. D. Hay. 1988. Development (Camb.). 102:605-622) that definitive epithelia can give rise to fibroblast-like cells when suspended within type I collagen gels. We wanted to know whether Madin-Darby canine kidney (MDCK) cells, an epithelial line, can form mesenchyme under similar conditions. Small explants of MDCK cells on basement membrane were suspended within or placed on top of extracellular matrix gels. MDCK cells on basement membrane gel are tall, columnar in shape, and ultrastructurally resemble epithelia transporting fluid and ions. MDCK explants cultured on type I collagen gel give rise to isolated fusiform-shaped cells that migrate over the gel surface. The fusiform cells extend pseudopodia and filopodia, lose cell membrane specializations, and develop an actin cortex around the entire cell. Unlike true mesenchymal cells, which express vimentin and type I collagen, fusiform cells produce both keratin and vimentin, continue to express laminin, and do not turn on type I collagen. Fusiform cells are not apically-basally polarized, but show mesenchymal cell polarity. Influenza hemagglutinin and virus budding localize to the front end or entire cell surface. Na,K-ATPase occurs intracellularly and also symmetrically distributes on the cell surface. Fodrin becomes diffusely distributed along the plasma membrane, ZO-1 cannot be detected, and desmoplakins distribute randomly in the cytoplasm. The loss of epithelial polarity and acquisition of mesenchymal cell polarity and shape by fusiform MDCK cells on type I collagen gel was previously unsuspected. The phenomenon may offer new opportunities for studying cytoplasmic and nuclear mechanisms regulating cell shape and polarity.

MeSH Terms
Animals Basement Membrane Cell Line Cell Membrane/analysis,enzymology,ultrastructure Collagen/biosynthesis,pharmacology Cytoplasm/ultrastructure Cytoskeletal Proteins Desmoplakins Epithelial Cells Epithelium/ultrastructure Extracellular Matrix Gels Hemagglutinins, Viral/analysis Influenza A virus/growth & development Intercellular Junctions/ultrastructure Intermediate Filaments/ultrastructure Membrane Glycoproteins/analysis Membrane Proteins/analysis Mesoderm/cytology Microvilli/ultrastructure Organelles/ultrastructure Pseudopodia/ultrastructure Sodium-Potassium-Exchanging ATPase/analysis
Chemicals
Cytoskeletal Proteins Desmoplakins Gels Hemagglutinins, Viral Membrane Glycoproteins Membrane Proteins Collagen Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zuk A
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
Matlin K S
Hay E D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.3.903
Published
1989-03-00
Pages
903-19
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115371
Subset
IM
Grants
NCI NIH HHS · 1F32-CA08380 · United States
NCI NIH HHS · R01-CA44331 · United States
NICHD NIH HHS · R01-HD00143 · United States
External Links
PubMed source DOI: 10.1083/jcb.108.3.903 PMC Full Text View journal details
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