Home LiteratureArticle Details
PMID: 2536092 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polyomavirus DNA is damaged in target cells during cytotoxic T-lymphocyte-mediated killing.

Journal of virology ·Vol. 63 ·No. 2 ·1989-02-00 ·Pages 572-8

Sellins KS, Cohen JJ

Abstract

Target cell DNA damage is an early event in cytotoxic T-lymphocyte (CTL)-mediated killing. It has been hypothesized that this DNA damage may serve as one mechanism of destroying viral genetic material inside infected cells. We directly examined the fate of viral DNA in target cells during CTL-mediated lysis. Polyomavirus DNA in transfected murine P815 mastocytoma targets was digested along with cellular DNA into oligonucleosome-sized fragments, although intact forms, possibly virion-associated DNA, were also present. In infected BALB/3T3 murine fibroblasts, which normally undergo single-stranded nicks when killed by CTL, polyomavirus DNA was converted to relaxed forms in the presence of CTL. These results suggest that the fate of the viral DNA depends on the stage of the viral life cycle and corresponds to the fate of the host cell DNA. Cleavage of the viral genome prior to assembly may thus be an important mechanism in specific antiviral immunity.

MeSH Terms
Animals Cytotoxicity, Immunologic DNA Damage DNA, Viral/genetics Fibroblasts/microbiology Hematopoietic Stem Cells/microbiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Polyomavirus/genetics T-Lymphocytes, Cytotoxic/immunology Tumor Cells, Cultured/microbiology
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sellins K S
Department of Microbiology and Immunology, University of Colorado School of Medicine, Denver 80262.
Cohen J J
References (33)
33 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. The degree of CTL-induced DNA solubilization is not determined by the human vs mouse origin of the target cell.
    J Immunol. 1987 Jun 1;138(11):3695-8 PMID: 3495567
  3. H-2 compatibility requirement for virus-specific T cell-mediated effector functions in vivo. I. Specificity of T cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with H-2K and H-2D.
    J Immunol. 1976 Nov;117(5 Pt 1):1495-502 PMID: 794412
  4. Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells.
    Cell. 1981 Mar;23(3):809-14 PMID: 6261957
  5. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  6. Early events in polyoma virus infection: attachment, penetration, and nuclear entry.
    J Virol. 1976 Aug;19(2):620-36 PMID: 183018
  7. The arrangement of nucleosomes in nucleoprotein complexes from polyoma virus and SV40.
    Cell. 1977 May;11(1):35-49 PMID: 194707
  8. Polyoma virus basic proteins.
    J Gen Virol. 1972 Feb;14(2):141-55 PMID: 4334780
  9. The recovery of mice from influenza A virus infection: adoptive transfer of immunity with influenza virus-specific cytotoxic T lymphocytes recognizing a common virion antigen.
    Scand J Immunol. 1978;8(5):413-20 PMID: 82995
  10. Chromatin structure: deduced from a minichromosome.
    Science. 1975 Mar 28;187(4182):1202-3 PMID: 17754289
  11. Structure of Simian virus 40 chromosomes in nuclei from infected monkey cells.
    J Mol Biol. 1978 Nov 15;125(4):491-510 PMID: 216806
  12. Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.
    J Virol. 1977 Mar;21(3):1113-20 PMID: 191638
  13. Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus.
    Nature. 1978 May 18;273(5659):238-9 PMID: 306072
  14. Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus and in vitro properties.
    J Virol. 1985 Feb;53(2):552-60 PMID: 3918175
  15. Apoptosis, lymphocytotoxicity and the containment of viral infections.
    Med Hypotheses. 1985 Dec;18(4):399-404 PMID: 3854167
  16. Lymphoid and other tissue-specific phenotypes of polyomavirus enhancer recombinants: positive and negative combinational effects on enhancer specificity and activity.
    Mol Cell Biol. 1986 Jun;6(6):2068-79 PMID: 3023917
  17. Comparison of nuclease digestion of polyoma virus nucleoprotein complex and mouse chromatin.
    J Virol. 1978 Jan;25(1):175-86 PMID: 202734
  18. Mechanisms of recovery from a generalized viral infection: mousepox. II. Passive transfer of recovery mechanisms with immune lymphoid cells.
    J Exp Med. 1971 May 1;133(5):1074-89 PMID: 4324300
  19. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  20. Cytoimmunotherapy for persistent virus infection reveals a unique clearance pattern from the central nervous system.
    Nature. 1986 May 15-21;321(6067):239-43 PMID: 3086743
  21. Role of T-cell function in recovery from murine influenza infection.
    Cell Immunol. 1979 Mar 15;43(2):341-51 PMID: 113108
  22. Mechanisms of immune lysis. III. Characterization of the nature and kinetics of the cytotoxic T lymphocyte-induced nuclear lesion in the target.
    J Immunol. 1982 May;128(5):2087-94 PMID: 6977583
  23. The recovery of mice from influenza virus infection: adoptive transfer of immunity with immune T lymphocytes.
    Scand J Immunol. 1978;7(5):389-97 PMID: 307273
  24. Differences in target cell DNA fragmentation induced by mouse cytotoxic T lymphocytes and natural killer cells.
    J Immunol. 1986 Sep 1;137(5):1442-7 PMID: 2943792
  25. DNA fragmentation in targets of CTL: an example of programmed cell death in the immune system.
    Adv Exp Med Biol. 1985;184:493-508 PMID: 2994413
  26. Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.
    J Virol. 1980 Jan;33(1):567-71 PMID: 6245253
  27. Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6361-5 PMID: 6312454
  28. Antiviral protection by virus-immune cytotoxic T cells: infected target cells are lysed before infectious virus progeny is assembled.
    J Exp Med. 1977 Mar 1;145(3):644-51 PMID: 233908
  29. Cytotoxic T cells specific for influenza virus-infected target cells.
    Immunology. 1977 Feb;32(2):151-9 PMID: 300355
  30. Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo.
    J Virol. 1984 Sep;51(3):682-6 PMID: 6332201
  31. Intracellular reovirus survives cytotoxic T lymphocyte-mediated lysis of its host cell.
    J Gen Virol. 1987 Nov;68 ( Pt 11):2899-907 PMID: 2824667
  32. Mechanisms of immune lysis. I. Physiological distinction between target cell death mediated by cytotoxic T lymphocytes and antibody plus complement.
    J Immunol. 1980 Mar;124(3):1100-5 PMID: 6965686
  33. Biological properties of an influenza A virus-specific killer T cell clone. Inhibition of virus replication in vivo and induction of delayed-type hypersensitivity reactions.
    J Exp Med. 1981 Aug 1;154(2):225-34 PMID: 6267157
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.2.572-578.1989
Published
1989-02-00
Pages
572-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247725
Subset
IM
Grants
NIAID NIH HHS · AI-11661 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.2.572-578.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2