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PMID: 2531370 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A group II intron in the Neurospora mitochondrial coI gene: nucleotide sequence and implications for splicing and molecular evolution.

Nucleic acids research ·Vol. 17 ·No. 22 ·1989-11-25 ·Pages 9087-99

Field DJ, Sommerfield A, Saville BJ, Collins RA

Abstract

The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J. Mol. Biol. 184: 413-428, 1985). Here we show that coI intron 1 is a typical Group II intron, the only one identified to date in Neurospora. The differential effect of the cyt18-1 mutation suggests that splicing of certain introns could be regulated independently of others by nuclear-encoded proteins. The intron contains a long open reading frame (ORF) resembling that of the Neurospora Mauriceville mitochondrial plasmid. The intron and plasmid ORFs share unusual features of codon usage that suggest both evolved outside of the Neurospora mitochondrial genetic system.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Exons Genes, Fungal Introns Molecular Sequence Data Mutation Neurospora/genetics Neurospora crassa/genetics Nucleic Acid Conformation Plasmids RNA Splicing Restriction Mapping Sequence Homology, Nucleic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Field D J
Department of Botany, University of Toronto, Ontario, Canada.
Sommerfield A
Saville B J
Collins R A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.22.9087
Published
1989-11-25
Pages
9087-99
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335116
Subset
IM
Databases
GENBANK
X14669
External Links
PubMed source DOI: 10.1093/nar/17.22.9087 PMC Full Text View journal details
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