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题目:: Generation of functional human pancreatic β cells in vitro.
作者:: Pagliuca(Felicia W),Millman(Jeffrey R),Gürtler(Mads),Segel(Michael),Van Dervort(Alana),Ryu(Jennifer Hyoje),Peterson(Quinn P),Greiner(Dale),Melton(Douglas A)
状态:: 发布时间2014-10-11 , 更新时间 2016-10-19
期刊:: Cell
摘要:: The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide β cells. Here, we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive β cells from hPSC in vitro. These stem-cell-derived β cells (SC-β) express markers found in mature β cells, flux Ca(2+) in response to glucose, package insulin into secretory granules, and secrete quantities of insulin comparable to adult β cells in response to multiple sequential glucose challenges in vitro. Furthermore, these cells secrete human insulin into the serum of mice shortly after transplantation in a glucose-regulated manner, and transplantation of these cells ameliorates hyperglycemia in diabetic mice.
语言:: eng
DOI:

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