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PMID: 2529540 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Loss of the MYC gene amplified in human HL-60 cells after treatment with inhibitors of poly(ADP-ribose) polymerase or with dimethyl sulfoxide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7442-5

Shima H, Nakayasu M, Aonuma S, Sugimura T, Nagao M

Abstract

In HL-60 cells, a human promyelocytic leukemia cell line, the human c-myc gene, designated MYC, is amplified about 16-fold. On differentiation of the HL-60 cells into granulocytes induced by several inhibitors of poly(ADP-ribose) polymerase [NAD+ poly(adenosine diphosphate D-ribose)ADP-D-ribosyltransferase, EC 2.4.2.30] including benzamide, nicotinamide, coumarin, and 4-hydroxyquinazoline or dimethyl sulfoxide, some MYC loss was observed. In contrast, benzoic acid, a noninhibitory analogue of benzamide, did not induce either granulocytic differentiation or loss of MYC. Loss of MYC seems to be associated with granulocytic differentiation because the time course of its loss was similar to that of appearance of nitroblue tetrazolium-positive cells, mature granulocytes, and its loss was not observed on differentiation of HL-60 cells into macrophages induced by phorbol 12-myristate 13-acetate or teleocidin. The loss of MYC is not the reason for the down regulation of MYC expression observed within 1 hr after addition of inducers, since the loss of MYC was not detected by 1-day treatment with inducers.

MeSH Terms
Benzamides/pharmacology Blotting, Southern Cell Differentiation/drug effects Cell Line Coumarins/pharmacology Dimethyl Sulfoxide/pharmacology Gene Amplification Humans Leukemia, Promyelocytic, Acute/genetics Niacinamide/pharmacology Nucleic Acid Hybridization Poly(ADP-ribose) Polymerase Inhibitors Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes/drug effects Quinazolines/pharmacology Quinazolinones RNA, Neoplasm/drug effects,genetics
Chemicals
Benzamides Coumarins Poly(ADP-ribose) Polymerase Inhibitors Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Quinazolines Quinazolinones RNA, Neoplasm Niacinamide benzamide 4-hydroxyquinazoline coumarin Dimethyl Sulfoxide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shima H
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Nakayasu M
Aonuma S
Sugimura T
Nagao M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7442
Published
1989-10-00
Pages
7442-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298080
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.19.7442 PMC Full Text View journal details
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