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PMID: 2525567 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis.

The Journal of clinical investigation ·Vol. 84 ·No. 1 ·1989-07-00 ·Pages 100-6

Giorgi D, Bernard JP, Rouquier S, Iovanna J, Sarles H, Dagorn JC

Abstract

The pancreatic stone protein and its secretory form (PSP-S) are inhibitors of CaCO3 crystal growth, possibly involved in the stabilization of pancreatic juice. We have established the structure of PSP-S mRNA and monitored its expression in chronic calcifying pancreatitis (CCP). A cDNA encoding pre-PSP-S has been cloned from a human pancreatic cDNA library. Its nucleotide sequence revealed that it comprised all but the 5' end of PSP-S mRNA, which was obtained by sequencing the first exon of the PSP-S gene. The complete mRNA sequence is 775 nucleotides long, including 5'- and 3'- noncoding regions of 80 and 197 nucleotides, respectively, attached to a poly(A) tail of approximately 125 nucleotides. It encodes a preprotein of 166 amino acids, including a prepeptide of 22 amino acids. No overall sequence homology was found between PSP-S and other pancreatic proteins. Some homology with several serine proteases was observed in the COOH-terminal region, however. The mRNA levels of PSP-S, trypsinogen, chymotrypsinogen, and colipase in CCP and control pancreas were compared. PSP-S mRNA was three times lower in CCP than in control, whereas the others were not altered. It was concluded that PSP-S gene expression is specifically reduced in CCP patients.

MeSH Terms
Adolescent Adult Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Blotting, Northern Calcium-Binding Proteins/genetics,metabolism Chronic Disease Chymotrypsinogen/genetics Colipases/genetics DNA/genetics Female Gene Expression Regulation Humans Lithostathine Male Molecular Sequence Data Nerve Tissue Proteins Nucleic Acid Hybridization Pancreatic Juice/analysis Pancreatitis/genetics,metabolism,pathology RNA, Messenger/genetics,metabolism Sequence Homology, Nucleic Acid Trypsinogen/genetics
Chemicals
Calcium-Binding Proteins Colipases Lithostathine Nerve Tissue Proteins REG1A protein, human RNA, Messenger Trypsinogen DNA Chymotrypsinogen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Giorgi D
Institut National de la Santé et de la Recherche Médicale, Marseille, France.
Bernard J P
Rouquier S
Iovanna J
Sarles H
Dagorn J C
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114128
Published
1989-07-00
Pages
100-6
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303958
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI114128 PMC Full Text View journal details
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