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PMID: 25240800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The RNA exosome promotes transcription termination of backtracked RNA polymerase II.

Nature structural & molecular biology ·Vol. 21 ·No. 10 ·2014-10-00 ·Pages 919-26

Lemay JF, Larochelle M, Marguerat S, Atkinson S, Bähler J, Bachand F

Abstract

The exosome is an RNA-decay complex that constantly monitors transcription and contributes to post-transcriptional turnover of faulty mRNAs. Yet how nuclear RNA surveillance by the exosome is coordinated with transcription is still unknown. Here we show that the RNA exosome of Schizosaccharomyces pombe can target the transcription machinery by terminating transcription events associated with paused and backtracked RNA polymerase II (RNAPII); this is contrary to the notion that the exosome acts exclusively on RNAs that have been released by RNAPII. Our data support a mechanism by which RNAPII backtracking provides a free RNA 3' end for the core exosome, which results in transcription termination with concomitant degradation of the associated transcript. These findings uncover a mechanism of cotranscriptional RNA surveillance whereby termination of transcription by the exosome prevents formation of aberrant readthrough RNAs and transcriptional interference at neighboring genes.

MeSH Terms
Exosome Multienzyme Ribonuclease Complex/genetics RNA Polymerase II/genetics,metabolism RNA Stability/genetics Schizosaccharomyces/genetics Transcription Termination, Genetic
Chemicals
RNA Polymerase II Exosome Multienzyme Ribonuclease Complex
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lemay Jean-François
1] RNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, Canada. [2].
Larochelle Marc
1] RNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, Canada. [2].
Marguerat Samuel
Medical Research Council Clinical Sciences Centre, Imperial College London, London, UK.
Atkinson Sophie
Department of Genetics, Evolution and Environment, University College London, London, UK.
Bähler Jürg ORCID
Department of Genetics, Evolution and Environment, University College London, London, UK.
Bachand François
RNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, Canada.
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.2893
Published
2014-10-00
Epub
2014-00-21
Pages
919-26
Language
English
Region
United States
NLM ID
101186374
Subset
IM
Grants
Wellcome Trust · 095598 · United Kingdom
Medical Research Council · MC_UP_1102/8 · United Kingdom
External Links
PubMed source DOI: 10.1038/nsmb.2893 View journal details
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