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题目:: Standing surface acoustic wave based cell coculture.
作者:: Li(Sixing),Guo(Feng),Chen(Yuchao),Ding(Xiaoyun),Li(Peng),Wang(Lin),Cameron(Craig E),Huang(Tony Jun)
状态:: 发布时间2014-10-07 , 更新时间 2016-10-19
期刊:: Anal Chem
摘要:: Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.
语言:: eng
DOI:

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