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PMID: 25135940 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

mRNA-programmed translation pauses in the targeting of E. coli membrane proteins.

eLife ·Vol. 3 ·2014-08-18

Fluman N, Navon S, Bibi E, Pilpel Y

Abstract

In all living organisms, ribosomes translating membrane proteins are targeted to membrane translocons early in translation, by the ubiquitous signal recognition particle (SRP) system. In eukaryotes, the SRP Alu domain arrests translation elongation of membrane proteins until targeting is complete. Curiously, however, the Alu domain is lacking in most eubacteria. In this study, by analyzing genome-wide data on translation rates, we identified a potential compensatory mechanism in E. coli that serves to slow down the translation during membrane protein targeting. The underlying mechanism is likely programmed into the coding sequence, where Shine-Dalgarno-like elements trigger elongation pauses at strategic positions during the early stages of translation. We provide experimental evidence that slow translation during targeting and improves membrane protein production fidelity, as it correlates with better folding of overexpressed membrane proteins. Thus, slow elongation is important for membrane protein targeting in E. coli, which utilizes mechanisms different from the eukaryotic one to control the translation speed.

Keywords
B. subtilis E. coli cell biology evolutionary biology genomics membrane proteins quality control translation rate
MeSH Terms
Bacillus subtilis/metabolism Cell Membrane/microbiology Codon Escherichia coli/metabolism Escherichia coli Proteins/chemistry Gene Expression Regulation, Bacterial Membrane Proteins/chemistry Mutagenesis Protein Biosynthesis Protein Structure, Tertiary Protein Transport Ribosomes/chemistry Signal Recognition Particle/chemistry
Chemicals
Codon Escherichia coli Proteins Membrane Proteins Signal Recognition Particle
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fluman Nir
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Navon Sivan
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Bibi Eitan
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
Pilpel Yitzhak
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
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Article Info
Journal
eLife
Abbr.
Elife
ISSN
2050-084X
Published
2014-08-18
Epub
2014-00-18
Language
English
Region
England
NLM ID
101579614
PMCID
PMC4359368
Subset
IM
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