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PMID: 2509073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Catecholaminergic innervation of GRF-containing neurons in the rat hypothalamus revealed by electron-microscopic cytochemistry.

Cell and tissue research ·Vol. 258 ·No. 1 ·1989-10-00 ·Pages 31-4

Sato A, Shioda S, Nakai Y

Abstract

The catecholaminergic innervation of neurons containing growth hormone-releasing factor (GRF) was examined by use of a method which combined either 5-hydroxydopamine (5-OHDA) uptake or autoradiography after intraventricular injection of 3H-noradrenaline with immunocytochemistry for GRF in the same tissue sections at the electron-microscopic level. In the ventrolateral part of the arcuate nucleus of the rat hypothalamus a large number of immunonegative axon terminals were found to make synaptic contact with GRF-like immunoreactive (GRF-LI) cell bodies and processes. 3H-noradrenaline autoradiography or 5-OHDA-labeling combined with GRF immunocytochemistry revealed that axon terminals labeled with 3H-noradrenaline or 5-OHDA make synaptic contact with the GRF-LI nerve cell bodies and processes. These findings indicate that catecholamine-containing neurons innervate GRF neurons to regulate GRF secretion via synapses in the rat arcuate nucleus.

MeSH Terms

Animals Arcuate Nucleus of Hypothalamus/analysis,cytology,ultrastructure Autoradiography Axons/ultrastructure Catecholamines/analysis Dendrites/ultrastructure Growth Hormone-Releasing Hormone/analysis Hydroxydopamines Immunohistochemistry Male Microscopy, Electron Neurons/analysis Norepinephrine Rats Rats, Inbred Strains

Chemicals

Catecholamines Hydroxydopamines 5-hydroxydopamine Growth Hormone-Releasing Hormone Norepinephrine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sato A

Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.

Shioda S

Nakai Y

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20 references, click to expand

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Article Info

Journal

Cell and tissue research

Abbr.

Cell Tissue Res

ISSN

0302-766X

DOI

10.1007/BF00223141

Published

1989-10-00

Pages

31-4

Language

English

Region

Germany

NLM ID

0417625

Subset

External Links

PubMed source DOI: 10.1007/BF00223141 View journal details

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Catecholaminergic innervation of GRF-containing neurons in the rat hypothalamus revealed by electron-microscopic cytochemistry.

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