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PMID: 2506136 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Beta interferon inhibits Toxoplasma gondii growth in human monocyte-derived macrophages.

Infection and immunity ·Vol. 57 ·No. 10 ·1989-10-00 ·Pages 3254-6

Schmitz JL, Carlin JM, Borden EC, Byrne GI

Abstract

Gamma interferon (IFN-gamma) previously has been shown to block the replication of Toxoplasma gondii in fibroblasts by the induction of indoleamine 2,3-dioxygenase (IDO) activity. IFN-beta also is known to induce IDO activity in monocyte-derived macrophages, but its ability to block the growth of T. gondii has not been demonstrated. We found not only that the combination of IFN-beta and lipopolysaccharide induced greater IDO activity in monocyte-derived macrophages than did IFN-beta alone but that this combination also was effective in inhibiting the growth of T. gondii. In addition, the inhibition was reversed by the addition of exogenous tryptophan, thus demonstrating that a mechanism by which IFN-beta inhibited T. gondii replication was by the induction of IDO.

MeSH Terms
Animals Enzyme Activation/drug effects Growth Inhibitors/pharmacology Humans Indoleamine-Pyrrole 2,3,-Dioxygenase Interferon Type I/pharmacology Interferon-gamma/pharmacology Lipopolysaccharides/pharmacology Macrophages/drug effects,enzymology,parasitology Monocytes/drug effects,enzymology,parasitology Oxygenases/metabolism,physiology Toxoplasma/drug effects,growth & development Tryptophan Oxygenase
Chemicals
Growth Inhibitors Indoleamine-Pyrrole 2,3,-Dioxygenase Interferon Type I Lipopolysaccharides Interferon-gamma Oxygenases Tryptophan Oxygenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schmitz J L
Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706.
Carlin J M
Borden E C
Byrne G I
References (13)
13 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.10.3254-3256.1989
Published
1989-10-00
Pages
3254-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260802
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.10.3254-3256.1989 PMC Full Text View journal details
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