Abstract
The development of intestinal polyps in an orthotopic colorectal mouse model, receiving a probiotic yogurt formulation containing microencapsulated live Lactobacillus acidophilus cells was investigated. The expression of various immunohistochemical markers namely CD8, Mac-1, Ki-67, and cleaved caspase-3, was evaluated. Results suggest that the probiotic formulation decreases overall intestinal inflammation. Mice receiving the probiotic formulation were found to develop almost two-fold fewer tumors in the small intestines. In the large intestine, however, there was no significant difference observed among polyp numbers. The formulation appears to have potential application in the prevention of various GI pathological conditions.
Keywords
GI health
artificial cell
inflammatory cytokines
intestinal polyps
microencapsulation
probiotics
MeSH Terms
Adenomatous Polyposis Coli/genetics,pathology,prevention & control
Adenomatous Polyposis Coli Protein/genetics,metabolism
Administration, Oral
Alginates/chemistry
Animals
CD8 Antigens/genetics,metabolism
Capsules/administration & dosage,chemistry
Carcinogenesis/drug effects,genetics,pathology
Caspase 3/genetics,metabolism
Cells, Immobilized/physiology
Disease Models, Animal
Gene Expression
Glucuronic Acid/chemistry
Hexuronic Acids/chemistry
Intestine, Large/drug effects,metabolism,pathology
Intestine, Small/drug effects,metabolism,pathology
Ki-67 Antigen/genetics,metabolism
Lactobacillus acidophilus/physiology
Macrophage-1 Antigen/genetics,metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Probiotics/therapeutic use
Chemicals
Adenomatous Polyposis Coli Protein
Alginates
CD8 Antigens
Capsules
Hexuronic Acids
Ki-67 Antigen
Macrophage-1 Antigen
Mki67 protein, mouse
Glucuronic Acid
Casp3 protein, mouse
Caspase 3
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Urbanska Aleksandra Malgorzata
a Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering and Physiology Artificial cells and Organs Research Center , Faculty of Medicine, McGill University , University Street, Montreal , QC , Canada.
Bhathena Jasmine
a Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering and Physiology Artificial cells and Organs Research Center , Faculty of Medicine, McGill University , University Street, Montreal , QC , Canada.
Cherif Sofiane
a Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering and Physiology Artificial cells and Organs Research Center , Faculty of Medicine, McGill University , University Street, Montreal , QC , Canada.
Prakash Satya
a Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering and Physiology Artificial cells and Organs Research Center , Faculty of Medicine, McGill University , University Street, Montreal , QC , Canada.
