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题目:: Establishment and Evaluation of an in vitro M Cell Model using C2BBe1 Cells and Raji Cells.
作者:: Masuda(Kazuya),Kajikawa(Akinobu),Igimi(Shizunobu)
状态:: 发布时间2014-07-21 , 更新时间 2014-07-28
期刊:: Biosci Microflora
摘要:: In vitro M cell models, consisting of co-cultures of Caco-2 cells and lymphoid cells, were developed and examined to observe bacterial transport. However, under our experimental conditions, the differentiation of Caco-2 cells into M cell-like cells could not be induced efficiently. To obtain a functionally stable M cell model based on human cells, C2BBe1 cells were screened and co-cultured with human Raji cells. In our co-cultures, increased sialyl Lewis A antigen expression and decreased Ulex europeaus agglutinin 1 binding were observed. Regarding the functional properties of the model, microsphere and lactic acid bacteria transport across the C2BBe1 co-cultures were increased compared with the levels seen in monocultures. The C2BBe1 monolayers that were co-cultured with Raji cells exhibited some M cell features; therefore, we consider our M cell model to be useful for investigating the interactions of bacteria with M cells.
语言:: eng
DOI:

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