| GeneLibs

题目:: Inhibition of B-cell proliferation and antibody production by mesenchymal stromal cells is mediated by T cells.
作者:: Rosado(Maria Manuela),Bernardo(Maria Ester),Scarsella(Marco),Conforti(Antonella),Giorda(Ezio),Biagini(Simone),Cascioli(Simona),Rossi(Francesca),Guzzo(Isabella),Vivarelli(Marina),Dello Strologo(Luca),Emma(Francesco),Locatelli(Franco),Carsetti(Rita)
状态:: 发布时间2014-12-24 , 更新时间 2016-01-01
期刊:: Stem Cells Dev
摘要:: Bone marrow (BM)-derived mesenchymal stromal cells (MSCs), endowed with immunosuppressive and anti-inflammatory properties, represent a promising tool in immunoregulatory and regenerative cell therapy. Clarifying the interactions between MSCs and B-lymphocytes may be crucial for designing innovative MSC-based strategies in conditions in which B cells play a role, including systemic lupus erythematosus (SLE) and rejection of kidney transplantation. In this study, we show that, both in healthy subjects and in patients, in vitro B-cell proliferation, plasma-cell differentiation, and antibody production are inhibited by BM-derived MSCs when peripheral blood lymphocytes are stimulated with CpG, but not when sorted B cells are cultured with MSCs+CpG. Inhibition is restored in CpG+MSC cocultures when sorted T cells are added to sorted B cells, suggesting that this effect is mediated by T cells, with both CD4(+) and CD8(+) cells playing a role. Moreover, cell-cell contact between MSCs and T cells, but not between MSCs and B cells, is necessary to inhibit B-cell proliferation. Thus, the presence of functional T cells, as well as cell-cell contact between MSCs and T cells, are crucial for B-cell inhibition. This information can be relevant for implementing MSC-based therapeutic immune modulation in patients in whom T-cell function is impaired.
语言:: eng
DOI:

文献库文献相关信息

DataTable pData

联系方式

微信公众号

文献库 文献相关信息

DataTable pData

联系方式

微信公众号

文献库文献相关信息