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PMID: 2501755 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a putative transcription factor gene expressed in the 20-OH-ecdysone inducible puff 74EF in Drosophila melanogaster.

Nucleic acids research ·Vol. 17 ·No. 12 ·1989-06-26 ·Pages 4455-64

Janknecht R, Taube W, Lüdecke HJ, Pongs O

Abstract

Drosophila melanogaster DNA has been cloned which encompasses the major part of the 20-OH-ecdysone inducible puff 74EF. One 20-OH-ecdysone responsive transcription unit was detected which gives rise to two alternative transcripts. The expression of one transcript in salivary glands of 3rd instar larvae is correlated with the 20-OH-ecdysone induced activity of puff 74EF. Corresponding cDNA analysis indicates that the two transcripts are translated into two different proteins which have alternative amino terminal ends. The carboxy terminal domain of the 74E proteins is similar to the carboxy terminal sequences of ets-oncoproteins suggesting that the 74E proteins represent alternative transcription factors. It is proposed that the activity of the 20-OH-ecdysone inducible puff 74EF leads to a switch in the synthesis of a transcription factor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular DNA/isolation & purification Drosophila Proteins Drosophila melanogaster/genetics,growth & development Ecdysone/physiology Gene Expression Regulation Insect Hormones/genetics,isolation & purification Molecular Sequence Data Peptide Elongation Factors/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Drosophila Proteins Eip74EF protein, Drosophila Insect Hormones Peptide Elongation Factors Transcription Factors Ecdysone DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Janknecht R
Lehrstuhl für Biochemie, Ruhr-Universität Bochum, FRG.
Taube W
Lüdecke H J
Pongs O
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.12.4455
Published
1989-06-26
Pages
4455-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318005
Subset
IM
Databases
GENBANK
X15087
External Links
PubMed source DOI: 10.1093/nar/17.12.4455 PMC Full Text View journal details
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