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PMID: 2499882 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

tRNA-tRNA interactions within cellular ribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 12 ·1989-06-00 ·Pages 4397-401

Smith D, Yarus M

Abstract

We describe an assay that converts the effects of tRNA-tRNA contacts at two particular codons into a quantitative effect on beta-galactosidase level. The assay measures the separate and combined efficiency of suppression at adjacent nonsense codons in vivo using a set of specially created homologous messages. In a survey of distal anticodon arm substitutions, we find that particular mutant tRNAs occupying the P-site reduce the apparent efficiency of the suppressor tRNA reading the A-site codon by factors of 2-170. By using measured tRNA-tRNA distances and the crystallographic tRNA structure, we propose a model of the tRNA-tRNA-mRNA complex. In the model, the anticodon loops of the P-site and A-site tRNAs contact one another in a way that is consistent with our combined tRNA efficiency data. These results suggest that tRNA-tRNA interactions that modulate tRNA action are an inevitable feature of translation.

MeSH Terms
Anticodon/genetics Base Sequence Codon/genetics Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA, Messenger/genetics RNA, Transfer/genetics,metabolism,ultrastructure Ribosomes/metabolism beta-Galactosidase/genetics
Chemicals
Anticodon Codon RNA, Messenger RNA, Transfer beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smith D
Department of Molecular, Cellular, University of Colorado, Boulder 80309-0347.
Yarus M
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17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.12.4397
Published
1989-06-00
Pages
4397-401
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287276
Subset
IM
Grants
NIGMS NIH HHS · GM30881 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.12.4397 PMC Full Text View journal details
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