Home LiteratureArticle Details
PMID: 2498085 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 5' flanking region of the pS2 gene contains a complex enhancer region responsive to oestrogens, epidermal growth factor, a tumour promoter (TPA), the c-Ha-ras oncoprotein and the c-jun protein.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 823-9

Nunez AM, Berry M, Imler JL, Chambon P

Abstract

Expression of the pS2 gene which is transcriptionally controlled by oestrogens in the breast cancer cell line MCF-7 is oestrogen independent in stomach mucosa. We show here that the level of MCF-7 cell pS2 mRNA can also be increased by the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). We further demonstrate, using transient transfection assays, that the -428 to -332 5' flanking sequence of the pS2 gene contains DNA enhancer elements responsive to oestrogens, TPA, EGF, the c-Ha-ras oncoprotein and the c-jun protein.

MeSH Terms
Base Sequence Breast Neoplasms/genetics,metabolism DNA, Neoplasm/genetics DNA-Binding Proteins/pharmacology Enhancer Elements, Genetic/drug effects Epidermal Growth Factor/pharmacology Estrogens/pharmacology Female Gene Expression Regulation/drug effects Humans Molecular Sequence Data Neoplasm Proteins/genetics Proteins Proto-Oncogene Proteins/pharmacology Proto-Oncogene Proteins c-jun Proto-Oncogene Proteins p21(ras) RNA, Messenger/genetics,metabolism RNA, Neoplasm/genetics,metabolism Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/pharmacology Trefoil Factor-1 Tumor Cells, Cultured/drug effects,metabolism Tumor Suppressor Proteins
Chemicals
DNA, Neoplasm DNA-Binding Proteins Estrogens Neoplasm Proteins Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-jun RNA, Messenger RNA, Neoplasm TFF1 protein, human Transcription Factors Trefoil Factor-1 Tumor Suppressor Proteins Epidermal Growth Factor HRAS protein, human Proto-Oncogene Proteins p21(ras) Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nunez A M
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médicine, Strasbourg, France.
Berry M
Imler J L
Chambon P
References (71)
71 references, click to expand
  1. A Harvey-ras responsive transcription element is also responsive to a tumour-promoter and to serum.
    Nature. 1988 Mar 17;332(6161):275-8 PMID: 2831462
  2. Activation of cell-specific expression of rat growth hormone and prolactin genes by a common transcription factor.
    Science. 1988 Mar 18;239(4846):1400-5 PMID: 2831625
  3. The 5' flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.
    Biochem Biophys Res Commun. 1988 Feb 29;151(1):306-13 PMID: 2450536
  4. Estrogens and growth factors induce the mRNA of the 52K-pro-cathepsin-D secreted by breast cancer cells.
    Nucleic Acids Res. 1988 Mar 25;16(5):1903-19 PMID: 3282224
  5. Independent molecular pathways in initiation and loss of hormone responsiveness of breast carcinomas.
    Science. 1988 Apr 22;240(4851):524-6 PMID: 3282307
  6. v-jun is a transcriptional activator, but not in all cell-lines.
    Nucleic Acids Res. 1988 Apr 11;16(7):3005-12 PMID: 2835749
  7. The 'CC.Ar.GG' box. A protein-binding site common to transcription-regulatory regions of the cardiac actin, c-fos and interleukin-2 receptor genes.
    Eur J Biochem. 1988 May 2;173(3):507-15 PMID: 3131140
  8. Transforming growth factor alpha.
    Cell. 1988 Aug 26;54(5):593-5 PMID: 3044605
  9. The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
    Nature. 1988 Aug 25;334(6184):661-5 PMID: 3045562
  10. Activation of the polyomavirus enhancer by a murine activator protein 1 (AP1) homolog and two contiguous proteins.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5839-43 PMID: 2842750
  11. Expression of transforming growth factor alpha and its messenger ribonucleic acid in human breast cancer: its regulation by estrogen and its possible functional significance.
    Mol Endocrinol. 1988 Jun;2(6):543-55 PMID: 3047554
  12. A surprising sequence homology.
    Biochem J. 1988 Jul 1;253(1):309 PMID: 3421950
  13. The N-terminal DNA-binding 'zinc finger' of the oestrogen and glucocorticoid receptors determines target gene specificity.
    EMBO J. 1988 Oct;7(10):3037-44 PMID: 3141145
  14. Transforming but not immortalizing oncogenes activate the transcription factor PEA1.
    EMBO J. 1988 Aug;7(8):2475-83 PMID: 3142763
  15. Induction of epidermal growth factor-related polypeptides by 17 beta-estradiol in MCF-7 human breast cancer cells.
    Endocrinology. 1986 Jan;118(1):138-42 PMID: 3000728
  16. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
    Nature. 1986 Mar 13-19;320(6058):134-9 PMID: 3754034
  17. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500 PMID: 3458212
  18. Multiple sequence motifs are involved in SV40 enhancer function.
    EMBO J. 1986 Feb;5(2):387-97 PMID: 3011406
  19. The immunoglobulin heavy-chain B-lymphocyte enhancer efficiently stimulates transcription in non-lymphoid cells.
    EMBO J. 1986 Mar;5(3):553-60 PMID: 3011412
  20. Secretion of an insulin-like growth factor-I-related protein by human breast cancer cells.
    Cancer Res. 1986 Sep;46(9):4613-9 PMID: 3731113
  21. Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors.
    Cell. 1986 Aug 15;46(4):567-74 PMID: 3524858
  22. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  23. Two different cis-active elements transfer the transcriptional effects of both EGF and phorbol esters.
    Science. 1986 Dec 19;234(4783):1552-7 PMID: 3491428
  24. Estrogen receptor in a human cell line (MCF-7) from breast carcinoma.
    J Biol Chem. 1973 Sep 10;248(17):6251-3 PMID: 4353636
  25. The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP.
    Genes Dev. 1988 Apr;2(4):394-402 PMID: 2453393
  26. Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters.
    Cell. 1988 Jul 29;54(3):325-34 PMID: 2840203
  27. Epidermal growth factor receptor gene expression in estrogen receptor-positive and negative human breast cancer cell lines.
    Mol Endocrinol. 1987 Mar;1(3):216-23 PMID: 3502607
  28. Multihormonal regulation of insulin-like growth factor-I-related protein in MCF-7 human breast cancer cells.
    Mol Endocrinol. 1988 Mar;2(3):200-8 PMID: 3041262
  29. Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa.
    Science. 1988 Aug 5;241(4866):705-8 PMID: 3041593
  30. The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes.
    Cell. 1988 Aug 12;54(4):541-52 PMID: 3135940
  31. fos-associated cellular p39 is related to nuclear transcription factor AP-1.
    Cell. 1988 Aug 12;54(4):553-60 PMID: 3135941
  32. Calcium and growth factor pathways of c-fos transcriptional activation require distinct upstream regulatory sequences.
    Mol Cell Biol. 1988 Jul;8(7):2787-96 PMID: 3136322
  33. Epidermal growth factor stimulates transcription of the c-jun proto-oncogene in rat fibroblasts.
    Nature. 1988 Aug 11;334(6182):538-9 PMID: 3136398
  34. Induction of proto-oncogene JUN/AP-1 by serum and TPA.
    Nature. 1988 Aug 18;334(6183):629-31 PMID: 2457172
  35. A human cell line from a pleural effusion derived from a breast carcinoma.
    J Natl Cancer Inst. 1973 Nov;51(5):1409-16 PMID: 4357757
  36. The inhibition of gastric acid secretion by epidermal growth factor.
    Experientia. 1975 Jul 15;31(7):825-6 PMID: 237781
  37. Isolation and structure of urogastrone and its relationship to epidermal growth factor.
    Nature. 1975 Sep 25;257(5524):325-7 PMID: 1161035
  38. The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture.
    Cancer Res. 1976 Dec;36(12):4595-601 PMID: 1000504
  39. Estrogen control of progesterone receptor in human breast cancer. Correlation with nuclear processing of estrogen receptor.
    J Biol Chem. 1978 Apr 10;253(7):2223-8 PMID: 632265
  40. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  41. Pancreatic spasmolytic polypeptide (PSP): III. Pharmacology of a new porcine pancreatic polypeptide with spasmolytic and gastric acid secretion inhibitory effects.
    Regul Pept. 1982 Mar;3(3-4):231-43 PMID: 6919177
  42. Effect of estradiol on human breast cancer cells in culture.
    Cancer Res. 1983 Jan;43(1):349-54 PMID: 6847777
  43. The SV40 72 bp repeat preferentially potentiates transcription starting from proximal natural or substitute promoter elements.
    Cell. 1983 Feb;32(2):503-14 PMID: 6297796
  44. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line.
    Nucleic Acids Res. 1982 Dec 20;10(24):7895-903 PMID: 6897676
  45. Clonal variation of MCF-7 breast cancer cells in vitro and in athymic nude mice.
    Cancer Res. 1983 May;43(5):2223-39 PMID: 6831445
  46. Plasmids for the cloning and expression of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter.
    Nucleic Acids Res. 1983 Oct 25;11(20):7119-36 PMID: 6314276
  47. Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7.
    Nucleic Acids Res. 1984 Mar 26;12(6):2861-78 PMID: 6324130
  48. Short and long range activation by the SV40 enhancer.
    Nucleic Acids Res. 1984 Jul 25;12(14):5589-608 PMID: 6087293
  49. New human transforming genes detected by a tumorigenicity assay.
    Mol Cell Biol. 1984 Sep;4(9):1695-705 PMID: 6092933
  50. Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6344-8 PMID: 6593703
  51. The amino acid sequence of pancreatic spasmolytic polypeptide.
    Biochim Biophys Acta. 1985 Mar 1;827(3):410-8 PMID: 2857575
  52. Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity.
    Science. 1985 May 10;228(4700):725-8 PMID: 4039465
  53. Evidence that transforming growth factor-beta is a hormonally regulated negative growth factor in human breast cancer cells.
    Cell. 1987 Feb 13;48(3):417-28 PMID: 2879636
  54. The c-Ha-ras oncogene and a tumor promoter activate the polyoma virus enhancer.
    Cell. 1987 Feb 13;48(3):525-34 PMID: 3026648
  55. Antiestrogen stimulation of the production of a 37,000 molecular weight secreted protein and estrogen stimulation of the production of a 32,000 molecular weight secreted protein in MCF-7 human breast cancer cells.
    Endocrinology. 1987 Mar;120(3):1140-51 PMID: 3803313
  56. Structure of the human oestrogen-responsive gene pS2.
    Nucleic Acids Res. 1987 Feb 25;15(4):1401-14 PMID: 3822834
  57. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  58. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  59. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  60. Synthesis and secretion of platelet-derived growth factor by human breast cancer cell lines.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5763-7 PMID: 3039506
  61. ras genes.
    Annu Rev Biochem. 1987;56:779-827 PMID: 3304147
  62. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen.
    Cell. 1987 Sep 11;50(6):847-61 PMID: 3040262
  63. Multiple cis- and trans-acting elements mediate the transcriptional response to phorbol esters.
    Nature. 1987 Oct 15-21;329(6140):648-51 PMID: 2821407
  64. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP.
    Cell. 1987 Oct 23;51(2):251-60 PMID: 2822255
  65. Characterization of the estrogen-induced pS2 protein secreted by the human breast cancer cell line MCF-7.
    Endocrinology. 1987 Nov;121(5):1759-65 PMID: 3665845
  66. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  67. Functional domains of the human estrogen receptor.
    Cell. 1987 Dec 24;51(6):941-51 PMID: 3690665
  68. Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9243-7 PMID: 3321071
  69. Effects of steroid hormones and peptide growth factors on protooncogene c-fos expression in human breast cancer cells.
    Cancer Res. 1988 Feb 15;48(4):802-5 PMID: 3257409
  70. v-jun encodes a nuclear protein with enhancer binding properties of AP-1.
    Cell. 1988 Mar 11;52(5):705-12 PMID: 2830989
  71. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-03-00
Pages
823-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400880
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2