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PMID: 2492403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interferon-gamma modulates messenger RNA levels of c-sis (PDGF-B chain), PDGF-A chain, and IL-1 beta genes in human vascular endothelial cells.

The American journal of pathology ·Vol. 134 ·No. 1 ·1989-01-00 ·Pages 35-43

Suzuki H, Shibano K, Okane M, Kono I, Matsui Y, Yamane K, Kashiwagi H

Abstract

The authors have investigated the effects of cytokines and lipopolysaccharide (LPS) on mRNA levels of c-sis (platelet-derived growth factor (PDGF)-B chain), PDGF-A chain, and interleukin 1 beta (IL-1 beta) genes in human vascular endothelial cells (EC). IL-1, tumor necrosis factor (TNF), and LPS not only enhanced the accumulation of c-sis mRNA, but also induced IL-1 beta gene expression. Interferon-gamma (IFN-gamma), in contrast, suppressed the accumulation of c-sis mRNA profoundly and PDGF-A chain mRNA to a lesser extent. The cytokine, in addition, suppressed the release of PDGF-like proteins by EC, while maintaining the growth of EC. IFN-gamma, however, augmented the levels of IL-1 beta mRNA in cultured EC in association with LPS or IL-1, suggesting that the suppression of c-sis expression was not mediated through modulation of IL-1 gene expression by IFN-gamma. These results raise the possibility that IFN-gamma may play a novel regulatory role in the pathogenesis of vascular diseases such as atherosclerosis and vasculitis.

MeSH Terms
Cells, Cultured Dose-Response Relationship, Drug Endothelium, Vascular/cytology,metabolism Gene Expression Regulation/drug effects Genes Humans Interferon-gamma/pharmacology,physiology Interleukin-1/genetics,pharmacology Platelet-Derived Growth Factor/genetics RNA, Messenger/metabolism Stereoisomerism Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Interleukin-1 Platelet-Derived Growth Factor RNA, Messenger Tumor Necrosis Factor-alpha Interferon-gamma
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Suzuki H
Department of Internal Medicine, University of Tsukuba, Japan.
Shibano K
Okane M
Kono I
Matsui Y
Yamane K
Kashiwagi H
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1989-01-00
Pages
35-43
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1879558
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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