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PMID: 2491838 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pathogenicity of Mycobacterium avium for human monocytes: absence of macrophage-activating factor activity of gamma interferon.

Infection and immunity ·Vol. 57 ·No. 1 ·1989-01-00 ·Pages 239-44

Toba H, Crawford JT, Ellner JJ

Abstract

Mycobacterium avium is a frequent opportunistic pathogen in the acquired immunodeficiency syndrome (AIDS). We compared 12 strains of M. avium in an in vitro model of pathogenicity. Peripheral blood-derived monocytes from healthy individuals were infected with M. avium in vitro. Bacterial uptake and intracellular replication were assessed by microscopic count of acid-fast bacilli and CFU of bacteria, respectively, in lysed monocytes. The CFU assay showed that among five AIDS-associated strains, only one replicated in monocytes. Two of seven non-AIDS-associated strains replicated intracellularly. In addition, we examined the effect of gamma interferon (IFN-gamma) on M. avium infection. IFN-gamma treatment of monocytes decreased phagocytosis and had no effect on the intracellular replication of M. avium. Thus, most strains of M. avium do not multiply within monocytes from healthy individuals and IFN-gamma does not have macrophage-activating factor activity for M. avium infection of human monocytes.

MeSH Terms
Acquired Immunodeficiency Syndrome/microbiology Colony Count, Microbial Humans Interferon-gamma/pharmacology Intracellular Fluid/drug effects,microbiology Lymphokines Macrophage-Activating Factors Monocytes/drug effects,microbiology Mycobacterium avium/drug effects,growth & development,pathogenicity
Chemicals
Lymphokines Macrophage-Activating Factors Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Toba H
Department of Medicine, Case Western Reserve University, Cleveland, Ohio.
Crawford J T
Ellner J J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.1.239-244.1989
Published
1989-01-00
Pages
239-44
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313080
Subset
IM
Grants
NIAID NIH HHS · AI 25799 · United States
External Links
PubMed source DOI: 10.1128/iai.57.1.239-244.1989 PMC Full Text View journal details
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