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PMID: 24874546 Published · ppublish English Case Reports Journal Article Research Support, N.I.H., Extramural

A novel de novo mutation of SCN8A (Nav1.6) with enhanced channel activation in a child with epileptic encephalopathy.

Neurobiology of disease ·Vol. 69 ·2014-09-00 ·Pages 117-23

Estacion M, O'Brien JE, Conravey A, Hammer MF, Waxman SG, Dib-Hajj SD, Meisler MH

Abstract

Rare de novo mutations of sodium channels are thought to be an important cause of sporadic epilepsy. The well established role of de novo mutations of sodium channel SCN1A in Dravet Syndrome supports this view, but the etiology of many cases of epileptic encephalopathy remains unknown. We sought to identify the genetic cause in a patient with early onset epileptic encephalopathy by whole exome sequencing of genomic DNA. The heterozygous mutation c. 2003C>T in SCN8A, the gene encoding sodium channel Nav1.6, was detected in the patient but was not present in either parent. The resulting missense substitution, p.Thr767Ile, alters an evolutionarily conserved residue in the first transmembrane segment of channel domain II. The electrophysiological effects of this mutation were assessed in neuronal cells transfected with mutant or wildtype cDNA. The mutation causes enhanced channel activation, with a 10mV depolarizing shift in voltage dependence of activation as well as increased ramp current. In addition, pyramidal hippocampal neurons expressing the mutant channel exhibit increased spontaneous firing with PDS-like complexes as well as increased frequency of evoked action potentials. The identification of this new gain-of-function mutation of Nav1.6 supports the inclusion of SCN8A as a causative gene in infantile epilepsy, demonstrates a novel mechanism for hyperactivity of Nav1.6, and further expands the role of de novo mutations in severe epilepsy.

Keywords
De novo mutation Epilepsy Epileptic encephalopathy Sodium channel
MeSH Terms
Action Potentials/physiology Age of Onset Animals DNA Mutational Analysis Epilepsy/genetics,physiopathology HEK293 Cells Hippocampus/physiopathology Humans Membrane Potentials/physiology Mutation, Missense NAV1.6 Voltage-Gated Sodium Channel/genetics,metabolism Neurons/physiology Patch-Clamp Techniques Pyramidal Cells/physiopathology Rats Rats, Sprague-Dawley Sequence Homology, Amino Acid Transfection
Chemicals
NAV1.6 Voltage-Gated Sodium Channel SCN8A protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Estacion Mark
The Center for Neuroscience & Regeneration Research, Yale School of Medicine, New Haven, CT 06520, USA; The Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, CT 06516, USA.
O'Brien Janelle E
Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109-5618, USA.
Conravey Allison
New Orleans Children's Hospital, New Orleans, LA 70118, USA.
Hammer Michael F
ARL Division of Biotechnology, University of Arizona, Tucson, AZ 85721, USA.
Waxman Stephen G
The Center for Neuroscience & Regeneration Research, Yale School of Medicine, New Haven, CT 06520, USA; The Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, CT 06516, USA.
Dib-Hajj Sulayman D
The Center for Neuroscience & Regeneration Research, Yale School of Medicine, New Haven, CT 06520, USA; The Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, CT 06516, USA. Electronic address: sulayman.dib-hajj@yale.edu.
Meisler Miriam H
Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109-5618, USA. Electronic address: meislerm@umich.edu.
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Article Info
Journal
Neurobiology of disease
Abbr.
Neurobiol Dis
ISSN
1095-953X
Published
2014-09-00
Epub
2014-00-27
Pages
117-23
Language
English
Region
United States
NLM ID
9500169
PMCID
PMC4124819
Subset
IM
Grants
NINDS NIH HHS · R01 NS034509 · United States
NIGMS NIH HHS · T32 GM007544 · United States
NINDS NIH HHS · R01 NS34509 · United States
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