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PMID: 2482734 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.

The Biochemical journal ·Vol. 264 ·No. 3 ·1989-12-15 ·Pages 765-9

Wuytack F, Eggermont JA, Raeymaekers L, Plessers L, Casteels R

Abstract

We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2 encoding the sarcoplasmic reticulum Ca2+ pump in slow skeletal and cardiac muscle. This alternatively spliced transcript was shown to be mainly represented in non-muscle tissues. These antibodies have enabled us to show the presence of the unique C-terminus of this type of Ca2+ pump, as predicted from the cDNA sequence, in the endoplasmic reticulum of vascular and gastric smooth muscle, liver and kidney.

MeSH Terms

Amino Acid Sequence Animals Antibodies Aorta/enzymology Calcium-Transporting ATPases/genetics,immunology Cattle Cell Membrane/enzymology Endoplasmic Reticulum/enzymology Epitopes/analysis Genes Isoenzymes/genetics,immunology Microsomes/enzymology Molecular Sequence Data Muscle, Smooth/enzymology Muscle, Smooth, Vascular/enzymology Muscles/enzymology Oligopeptides/chemical synthesis Peptide Fragments/analysis RNA Splicing Rabbits/immunology Stomach/enzymology Swine Transcription, Genetic

Chemicals

Antibodies Epitopes Isoenzymes Oligopeptides Peptide Fragments Calcium-Transporting ATPases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Wuytack F

Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium.

Eggermont J A

Raeymaekers L

Plessers L

Casteels R

References (18)

18 references, click to expand

Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+-ATPase mRNA.
FEBS Lett. 1989 May 22;249(1):35-41 PMID: 2542094
Molecular cloning of the mammalian smooth muscle sarco(endo)plasmic reticulum Ca2+-ATPase.
J Biol Chem. 1989 Apr 25;264(12):7059-65 PMID: 2523389
Phosphorylated intermediates of (Ca2+ + Mg2+)-ATPase and alkaline phosphatase in renal plasma membranes.
Biochim Biophys Acta. 1983 Mar 9;728(3):409-18 PMID: 6130791
Preparation of rough microsomes and membrane-bound polysomes that are active in protein synthesis in vitro.
Methods Enzymol. 1983;96:3-24 PMID: 6656633
Subcellular fractionation of pig stomach smooth muscle. A study of the distribution of the (Ca2+ + Mg2+)-ATPase activity in plasmalemma and endoplasmic reticulum.
Biochim Biophys Acta. 1985 May 28;815(3):441-54 PMID: 3158351
Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence.
Nature. 1985 Aug 22-28;316(6030):696-700 PMID: 2993904
Two Ca2+ ATPase genes: homologies and mechanistic implications of deduced amino acid sequences.
Cell. 1986 Feb 28;44(4):597-607 PMID: 2936465
Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions.
Anal Biochem. 1986 Mar;153(2):267-71 PMID: 3706710
Characterization of heart cytosolic proteins capable of modulating calcium uptake by the sarcoplasmic reticulum. 1. Isolation of a protein with protective activity and its identification as muscle albumin.
Eur J Biochem. 1987 Jan 15;162(2):365-70 PMID: 2948823
Adult forms of the Ca2+ATPase of sarcoplasmic reticulum. Expression in developing skeletal muscle.
J Biol Chem. 1987 Mar 15;262(8):3768-74 PMID: 3029125
(Ca2+ + Mg2+)-dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles.
FEBS Lett. 1988 Feb 29;229(1):45-8 PMID: 2831089
Structure of the rabbit fast-twitch skeletal muscle Ca2+-ATPase gene.
J Biol Chem. 1988 Apr 5;263(10):4813-9 PMID: 2965149
A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study.
Cell Motil Cytoskeleton. 1988;9(2):164-74 PMID: 2965994
Molecular cloning of cDNAs from human kidney coding for two alternatively spliced products of the cardiac Ca2+-ATPase gene.
J Biol Chem. 1988 Oct 15;263(29):15024-31 PMID: 2844796
A novel Ca2+ pump expressed in brain, kidney, and stomach is encoded by an alternative transcript of the slow-twitch muscle sarcoplasmic reticulum Ca-ATPase gene. Identification of cDNAs encoding Ca2+ and other cation-transporting ATPases using an oligonucleotide probe derived from the ATP-binding site.
J Biol Chem. 1988 Oct 15;263(29):15032-40 PMID: 2844797
Smooth muscle expresses a cardiac/slow muscle isoform of the Ca2+-transport ATPase in its endoplasmic reticulum.
Biochem J. 1989 Jan 1;257(1):117-23 PMID: 2521998
Molecular cloning and characterization of a Ca2+ + Mg2+-dependent adenosine triphosphatase from rat cardiac sarcoplasmic reticulum. Regulation of its expression by pressure overload and developmental stage.
J Clin Invest. 1989 Apr;83(4):1102-8 PMID: 2522936
Evidence for two isoforms of the endoplasmic-reticulum Ca2+ pump in pig smooth muscle.
Biochem J. 1989 Jun 15;260(3):757-61 PMID: 2527496

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2640765

Published

1989-12-15

Pages

765-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1133651

Subset

External Links

PubMed source DOI: 10.1042/bj2640765 PMC Full Text View journal details

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Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.

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