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PMID: 2481312 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Charybdotoxin inhibits proliferation and interleukin 2 production in human peripheral blood lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 10171-5

Price M, Lee SC, Deutsch C

Abstract

We demonstrate that blockade of the lymphocyte voltage-gated K+ channel by charybdotoxin (CTX) inhibits lymphocyte mitogenesis. Charybdotoxin blocks conductance with a Ki of 0.3 nM and inhibits mitogen- and antigen-stimulated proliferation with a Ki of 0.5 nM. As opposed to the other blockers of the lymphocyte K+ channel, the inhibition of mitogenesis by CTX can be overcome selectively by exogenous recombinant interleukin 2 (IL-2); endogenous levels of IL-2 in the culture supernatants of stimulated cells are decreased by the presence of CTX. Our results suggest that the voltage-gated K+ channel is either directly or indirectly involved in IL-2 synthesis and/or secretion.

MeSH Terms

4-Aminopyridine/pharmacology Cells, Cultured Charybdotoxin Humans Interleukin-2/biosynthesis Kinetics Lymphocyte Activation/drug effects Lymphocytes/drug effects,immunology Quinine/pharmacology Scorpion Venoms/pharmacology T-Lymphocytes/drug effects,immunology Tetraethylammonium Tetraethylammonium Compounds/pharmacology

Chemicals

Interleukin-2 Scorpion Venoms Tetraethylammonium Compounds Charybdotoxin Tetraethylammonium Quinine 4-Aminopyridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Price M

Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085.

Lee S C

Deutsch C

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.24.10171

Published

1989-12-00

Pages

10171-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298669

Subset

Grants

NIGMS NIH HHS · GM 41467 · United States

External Links

PubMed source DOI: 10.1073/pnas.86.24.10171 PMC Full Text View journal details

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Charybdotoxin inhibits proliferation and interleukin 2 production in human peripheral blood lymphocytes.

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