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题目:: Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system.
作者:: Asadi(Mohammad Hossein),Javanmardi(Setareh),Movahedin(Mansoureh)
状态:: 发布时间2014-05-06 , 更新时间 2014-07-31
期刊:: Iran J Reprod Med
摘要:: Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.,This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis Materials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively.,The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated signiﬁcant increase in pluripotency genes expression and signiﬁcant decrease in germ cell-speciﬁc genes expression.,The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells. This article extracted from Ph.D. thesis. (Setareh Javanmardi).
语言:: eng
DOI:

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