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PMID: 2477549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Single chloride-selective channel from cardiac sarcoplasmic reticulum studied in planar lipid bilayers.

The Journal of membrane biology ·Vol. 110 ·No. 1 ·1989-08-00 ·Pages 39-47

Rousseau E

Abstract

The behavior of single Cl- channel was studied by fusing isolated canine cardiac sarcoplasmic reticulum (SR) vesicles into planar lipid bilayers. The channel exhibited unitary conductance of 55 pS (in 260 mM Cl-) and steady-state activation. Subconductance states were observed. Open probability was dependent on holding potentials (-60 to +60 mV) and displayed a bell-shaped relationship, with probability values ranging from 0.2 to 0.8 with a maximum at -10 mV. Channel activity was irreversibly inhibited by DIDS, a stilbene derivative. Time analysis revealed the presence of one time constant for the full open state and three time constants for the closed states. The open and the longer closed time constants were found to be voltage dependent. The behavior of the channel was not affected by changing Ca2+ and Mg2+ concentrations in both chambers, nor by adding millimolar adenosine triphosphate, or by changing the pH from 7.4 to 6.8. The presence of sulfate anions decreased the unit current amplitude, but did not affect the open probability. These results reveal that at the unitary level the cardiac SR anion-selective channel has distinctive as well as similar electrical properties characteristic of other types of Cl- channels.

MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/pharmacology Animals Chlorides/metabolism,pharmacokinetics Dogs Electric Conductivity Intracellular Membranes/analysis,physiology,ultrastructure Ion Channels/metabolism,physiology,ultrastructure Lipids/analysis Membrane Potentials/drug effects Membranes/metabolism,physiology,ultrastructure Myocardium/cytology,ultrastructure Sarcoplasmic Reticulum/analysis,physiology,ultrastructure
Chemicals
Chlorides Ion Channels Lipids 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rousseau E
Department of Biochemistry and Nutrition, University of North Carolina, Chapel Hill 27599.
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42 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870991
Published
1989-08-00
Pages
39-47
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NHLBI NIH HHS · HL 27430 · United States
NHLBI NIH HHS · HL 38835 · United States
External Links
PubMed source DOI: 10.1007/BF01870991 View journal details
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