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PMID: 2476282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

5-Azadeoxycytidine induced undercondensation in the giant X chromosomes of Microtus agrestis.

Chromosoma ·Vol. 98 ·No. 2 ·1989-08-00 ·Pages 93-8

Haaf T, Schmid M

Abstract

Fibroblasts of female Microtus agrestis were treated with 5-azadeoxycytidine (5-aza-dCyd) at a final concentration of 10(-5) M during the last 2 h of culture. This cytidine analogue induces distinct undercondensation of the constitutive heterochromatin in the giant X chromosomes. The undercondensed heterochromatic thread exhibits longitudinal segmentation reminiscent of a chromomere pattern. In the late-replicating X chromosome, 5-aza-dCyd also inhibits condensation of the genetically inactivated euchromatin (facultative heterochromatin). The described effects of 5-aza-dCyd on the X chromosome structure appear to be incorporation independent.

MeSH Terms
Animals Arvicolinae/genetics Azacitidine/analogs & derivatives,pharmacology Cell Line DNA Replication Decitabine Female Fibroblasts Heterochromatin/metabolism Karyotyping Metaphase Translocation, Genetic X Chromosome/drug effects,metabolism,ultrastructure
Chemicals
Heterochromatin Decitabine Azacitidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haaf T
Department of Human Genetics, University of Würzburg, Federal Republic of Germany.
Schmid M
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20 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00291043
Published
1989-08-00
Pages
93-8
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00291043 View journal details
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