Caldesmon150, caldesmon77 and skeletal muscle troponin T share a common antigenic determinant.

FEBS letters ·Vol. 251 ·No. 1-2 ·1989-07-17 ·Pages 65-8

Shirinsky VP, Biryukov KG, Vorotnikov AV, Gusev NB

Abstract

Caldesmon and troponin are known to control actin-myosin interaction in smooth and striated muscle. We showed that polyclonal antibodies to smooth muscle caldesmon150 cross-react with non-muscle caldesmon77 and with skeletal muscle troponin T. The epitope recognized by caldesmon antibodies is located in the CB 2 peptide [residues 71-151] of troponin T. It is supposed that the observed cross-reactivity is due to the common structure of the tropomyosin-binding site of caldesmon and troponin T.

MeSH Terms

Animals Antibody Specificity Calmodulin-Binding Proteins/immunology Cattle Chickens Chymotrypsin Cyanogen Bromide Electrophoresis, Polyacrylamide Gel Epitopes/immunology Immunoblotting Molecular Weight Muscle, Smooth/analysis Muscles/analysis Peptide Fragments/immunology Rabbits Rats Troponin/immunology Troponin T

Chemicals

Calmodulin-Binding Proteins Epitopes Peptide Fragments Troponin Troponin T Chymotrypsin Cyanogen Bromide

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Shirinsky V P

Institute of Experimental Cardiology, USSR Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.

Biryukov K G

Vorotnikov A V

Gusev N B

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(89)81429-2

Published

1989-07-17

Pages

65-8

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(89)81429-2 View journal details

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Caldesmon150, caldesmon77 and skeletal muscle troponin T share a common antigenic determinant.

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