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PMID: 2473160 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of connective tissue-type mast cell proliferation by crosslinking of cell-bound IgE.

The Journal of experimental medicine ·Vol. 170 ·No. 1 ·1989-07-01 ·Pages 233-44

Takagi M, Nakahata T, Koike K, Kobayashi T, Tsuji K, Kojima S, Hirano T, Miyajima A, Arai K, Akabane T

Abstract

Crosslinking of cell-bound IgE on mouse connective tissue-type mast cells (CTMC) by multivalent antigen or anti-IgE antibody induced clonal growth of CTMC in methylcellulose culture containing IL-3. Continuous presence of antigen, IgE antibody, and IL-3 in culture was required for extensive proliferation of CTMC. Optimal concentrations of antigen and anti-IgE antibody for proliferation of sensitized CTMC approximately corresponded to those for maximal histamine release from the cells, and it was observed that most dividing cells stimulated by antigen had pericellular degranulation halos in culture. Experiments of both single cell culture and serum free culture provided evidence for a direct effect of antigen stimulation on proliferation of CTMC. Neither accessory cells nor some factors in FCS were required for the clonal growth of CTMC in our culture condition. Compound 48/80, a direct stimulator of CTMC, also triggered histamine release from CTMC but failed to support their proliferation. These results suggest that stimulation of CTMC via IgE receptors not only triggers the release of chemical mediators from the cells but induces clonal growth of CTMC in the presence of IL-3. Our data indicate the possibility that antigen stimulation may play another role in the proliferation of CTMC.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Differentiation, B-Lymphocyte/immunology Cell Division/drug effects Cells, Cultured Clone Cells Connective Tissue/immunology Connective Tissue Cells Histamine Release Immunoglobulin E/physiology Interleukin-3/pharmacology Male Mast Cells/cytology,immunology,physiology Mice Mice, Inbred Strains Receptors, Fc/immunology Receptors, IgE p-Methoxy-N-methylphenethylamine/pharmacology
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Antigens, Differentiation, B-Lymphocyte Interleukin-3 Receptors, Fc Receptors, IgE Immunoglobulin E p-Methoxy-N-methylphenethylamine
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Takagi M
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Nakahata T
Koike K
Kobayashi T
Tsuji K
Kojima S
Hirano T
Miyajima A
Arai K
Akabane T
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39 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.1.233
Published
1989-07-01
Pages
233-44
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189385
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.170.1.233 PMC Full Text View journal details
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