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PMID: 2472961 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutations in 16S ribosomal RNA disrupt antibiotic--RNA interactions.

The EMBO journal ·Vol. 8 ·No. 4 ·1989-04-00 ·Pages 1213-6

De Stasio EA, Moazed D, Noller HF, Dahlberg AE

Abstract

Two of six mutations at a base-paired site in Escherichia coli 16S rRNA confer resistance to nine different aminoglycoside antibiotics in vivo. Chemical probing of mutant and wild-type ribosomes in the presence of paromomycin indicates that interactions between the antibiotic and 16S rRNA in mutant ribosomes are disrupted. The altered interactions measured in vitro correlate precisely with resistance seen in vivo and may be attributable to specific structural changes observed in the mutant rRNA.

MeSH Terms

Aminoglycosides Anti-Bacterial Agents/pharmacology Base Composition Base Sequence Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Mutation RNA, Bacterial/genetics RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics

Chemicals

Aminoglycosides Anti-Bacterial Agents RNA, Bacterial RNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

De Stasio E A

Section of Biochemistry, Brown University, Providence, RI 02912.

Moazed D

Noller H F

Dahlberg A E

References (14)

14 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1989-04-00

Pages

1213-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC400937

Subset

Grants

NIGMS NIH HHS · GM 17129 · United States

NIGMS NIH HHS · GM 19756 · United States

External Links

PubMed source PMC Full Text View journal details

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Mutations in 16S ribosomal RNA disrupt antibiotic--RNA interactions.

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