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PMID: 2469081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Toxoplasma gondii: antigenic differences between endozoites and cystozoites defined by monoclonal antibodies.

Parasitology research ·Vol. 75 ·No. 3 ·1989-00-00 ·Pages 189-93

Omata Y, Igarashi M, Ramos MI, Nakabayashi T

Abstract

The antigenic differences between endozoites and cystozoites of Toxoplasma gondii were analyzed by indirect immunofluorescent assay and Western blotting using monoclonal antibodies. An antigenic determinant of an antigen of 20 kDa mol. wt. that appeared to be localized in the cytoplasm was observed to be specific to cystozoites alone. The 20-kDa antigen was diffused throughout the parasite and was observed in cystozoites produced both in vivo and in vitro.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Protozoan/analysis Blotting, Western Electrophoresis, Polyacrylamide Gel Epitopes/analysis Fluorescent Antibody Technique Hybridomas Mice Mice, Inbred BALB C Molecular Weight Toxoplasma/immunology
Chemicals
Antibodies, Monoclonal Antigens, Protozoan Epitopes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Omata Y
Department of Protozoology, Osaka University, Japan.
Igarashi M
Ramos M I
Nakabayashi T
References (12)
12 references, click to expand
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Article Info
Journal
Parasitology research
Abbr.
Parasitol Res
ISSN
0932-0113
DOI
10.1007/BF00931274
Published
1989-00-00
Pages
189-93
Language
English
Region
Germany
NLM ID
8703571
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00931274 View journal details
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