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PMID: 2465552 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Low density lipoprotein undergoes oxidative modification in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 4 ·1989-02-00 ·Pages 1372-6

Palinski W, Rosenfeld ME, Ylä-Herttuala S, Gurtner GC, Socher SS, Butler SW, Parthasarathy S, Carew TE, Steinberg D, Witztum JL

Abstract

It has been proposed that low density lipoprotein (LDL) must undergo oxidative modification before it can give rise to foam cells, the key component of the fatty streak lesion of atherosclerosis. Oxidation of LDL probably generates a broad spectrum of conjugates between fragments of oxidized fatty acids and apolipoprotein B. We now present three mutually supportive lines of evidence for oxidation of LDL in vivo: (i) Antibodies against oxidized LDL, malondialdehyde-lysine, or 4-hydroxynonenal-lysine recognize materials in the atherosclerotic lesions of LDL receptor-deficient rabbits; (ii) LDL gently extracted from lesions of these rabbits is recognized by an antiserum against malondialdehyde-conjugated LDL; (iii) autoantibodies against malondialdehyde-LDL (titers from 512 to greater than 4096) can be demonstrated in rabbit and human sera.

MeSH Terms
Alkaline Phosphatase/metabolism Animals Antibodies Antigen-Antibody Complex/analysis Aorta, Thoracic/metabolism,pathology Arteriosclerosis/metabolism,pathology Autoantibodies/immunology Epitopes/analysis Guinea Pigs Humans Hyperlipidemias/metabolism Immunoenzyme Techniques Lipoproteins, LDL/blood,immunology,metabolism Male Malonates/analysis Malondialdehyde/analysis,immunology Mice Oxidation-Reduction Rabbits Radioimmunoassay Reference Values
Chemicals
Antibodies Antigen-Antibody Complex Autoantibodies Epitopes Lipoproteins, LDL Malonates Malondialdehyde Alkaline Phosphatase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Palinski W
Department of Medicine, University of California, San Diego, La Jolla 92093.
Rosenfeld M E
Ylä-Herttuala S
Gurtner G C
Socher S S
Butler S W
Parthasarathy S
Carew T E
Steinberg D
Witztum J L
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.4.1372
Published
1989-02-00
Pages
1372-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286692
Subset
IM
Grants
NHLBI NIH HHS · HL14197 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.4.1372 PMC Full Text View journal details
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