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PMID: 2463631 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential effects of human papillomavirus type 6, 16, and 18 DNAs on immortalization and transformation of human cervical epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 2 ·1989-01-00 ·Pages 563-7

Pecoraro G, Morgan D, Defendi V

Abstract

The human papillomaviruses (HPVs) are associated with specific benign and malignant lesions of the skin and mucosal epithelia. Cloned viral DNAs from HPV types 6b, 16, and 18 associated with different pathological manifestations of genital neoplasia in vivo were introduced into primary human cervical epithelial cells by electroporation. Cells transfected with HPV16 or HPV18 DNA acquired indefinite lifespans, distinct morphological alterations, and anchorage-independent growth (HPV18), and contain integrated transcriptionally active viral genomes. HPV6b or plasmid electroporated cells senesced at low passage. The alterations in growth and differentiation of the cells appear to reflect the progressive oncogenic processes that result in cervical carcinoma in vivo.

MeSH Terms
Adult Blotting, Northern Blotting, Southern Cell Division Cell Transformation, Neoplastic Cell Transformation, Viral Cells, Cultured Cervix Uteri/cytology,microbiology DNA, Viral/physiology Epithelial Cells Epithelium/microbiology Female Humans Papillomaviridae/genetics Plasmids RNA/genetics Transcription, Genetic Transfection
Chemicals
DNA, Viral RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pecoraro G
Department of Pathology and Kaplan Cancer Center, New York University Medical Center, NY 10016.
Morgan D
Defendi V
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.2.563
Published
1989-01-00
Pages
563-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286512
Subset
IM
Grants
NCI NIH HHS · CA16239 · United States
PHS HHS · T32-09161 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.2.563 PMC Full Text View journal details
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