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PMID: 2461293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of two different genes (cDNA) for cytochrome c oxidase subunit VIa from heart and liver of the rat.

The EMBO journal ·Vol. 7 ·No. 8 ·1988-08-00 ·Pages 2387-91

Schlerf A, Droste M, Winter M, Kadenbach B

Abstract

By antibody screening of a rat liver and a rat heart cDNA library in lambda gt11 two clones coding for the liver- and heart-specific subunit VIa of rat cytochrome c oxidase were isolated. In the heart cDNA sequence a TAA stop codon was found in frame 18 bp 5' upstream of the first methionine codon, thus excluding a leader sequence for this protein. The two cDNAs contain the full-length coding region of two subunits. The amino acid sequences of the two subunits show only 50% homology, whereas 74% homology was found between rat heart and bovine heart subunit VIa. By Northern blot analysis it is shown that the gene for subunit VIa from heart is only expressed in heart and skeletal muscle, whereas that from liver is also expressed in kidney, brain, heart and weakly in muscle.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular Codon/genetics DNA/genetics Electron Transport Complex IV/genetics Liver/enzymology Molecular Sequence Data Myocardium/enzymology Protein Sorting Signals/genetics RNA/analysis Rats Sequence Homology, Nucleic Acid
Chemicals
Codon Protein Sorting Signals RNA DNA Electron Transport Complex IV
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schlerf A
Biochemie, Fb Chemie der Philipps-Universität, Marburg, FRG.
Droste M
Winter M
Kadenbach B
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-08-00
Pages
2387-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457104
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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