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PMID: 2460265 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) in Chlamydomonas reinhardtii.

Current genetics ·Vol. 14 ·No. 2 ·1988-08-00 ·Pages 119-26

Silk GW, Wu M

Abstract

A plasmid library of chloroplast (Cp) DNA from Chlamydomonas reinhardtii was used to screen for transcripts which respond to light. A transcript of R03, a 1,300 bp EcoRI fragment, was identified as a message which accumulates in darkness. The transcribed region of R03 showed extensive sequence homology with the Escherichia coli elongation factor gene, tufA. A gene-specific probe was constructed. Northern blots were used to study the extent and kinetics of accumulation of this transcript in darkness and in the presence of antibiotic inhibitors of Cp ribosomes. For comparison, the effects of darkness and antibiotics on the steady state levels of psbA, rbcL, and 16S rRNA were also studied. We conclude that the tuf transcript shows the greatest increase in darkness and in the presence of antibiotics.

MeSH Terms
Anti-Bacterial Agents/pharmacology Blotting, Northern Cell Cycle Chlamydomonas/drug effects,genetics,radiation effects Chloramphenicol/pharmacology Chloroplasts DNA/genetics Deoxyribonuclease EcoRI Light Nucleic Acid Hybridization Peptide Elongation Factor Tu/genetics RNA/genetics RNA Probes RNA, Ribosomal, 16S/drug effects,genetics,radiation effects Streptomycin/pharmacology Templates, Genetic Transcription, Genetic
Chemicals
Anti-Bacterial Agents RNA Probes RNA, Ribosomal, 16S RNA Chloramphenicol DNA Deoxyribonuclease EcoRI Peptide Elongation Factor Tu Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Silk G W
Department of Biological Sciences, University of Maryland Baltimore County, Catonsville 21228.
Wu M
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19 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00569335
Published
1988-08-00
Pages
119-26
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00569335 View journal details
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