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PMID: 2459839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Villin, intestinal brush border hydrolases and keratin polypeptides in intestinal metaplasia and gastric cancer; an immunohistologic study emphasizing the different degrees of intestinal and gastric differentiation in signet ring cell carcinomas.

Virchows Archiv. A, Pathological anatomy and histopathology ·Vol. 413 ·No. 4 ·1988-00-00 ·Pages 303-12

Osborn M, Mazzoleni G, Santini D, Marrano D, Martinelli G, Weber K

Abstract

Gastric carcinomas have been assayed for the presence of villin and for the small intestinal hydrolases aminopeptidase N and sucrase isomaltase. These proteins seem not to be present in normal stomach epithelium. However intestinal metaplasia in stomach, and tumour cells in the glandular patterns of gastric carcinoma were positive for all three markers, showing characteristic apical positivity. In contrast, in diffuse gastric carcinomas the percentage of signet ring cells positive for these markers varied from 10-100% with each marker showing a similar percentage of positive cells. Testing of gastric carcinomas with antibodies specific for different keratin polypeptides showed that while all 7 tumours were positive for keratins 8 and 18.2 were also positive for keratin 7. In the keratin 7 positive tumours all tumour cells were keratin 7 positive. The keratin 8 antibody also reacted on routinely fixed specimens. Thus gastric carcinomas reveal different degrees of gastric and intestinal differentiation.

MeSH Terms
Adenocarcinoma, Mucinous/analysis,enzymology Alkaline Phosphatase/analysis Aminopeptidases/analysis CD13 Antigens Calcium-Binding Proteins/analysis Carrier Proteins/analysis Fluorescent Antibody Technique Frozen Sections Humans Hydrolases/analysis Immunohistochemistry Intestine, Small/analysis,enzymology,pathology Keratins/analysis Metaplasia Microfilament Proteins/analysis Microvilli/analysis,enzymology Stomach Neoplasms/analysis,enzymology Sucrase-Isomaltase Complex/analysis
Chemicals
Calcium-Binding Proteins Carrier Proteins Microfilament Proteins villin Keratins Hydrolases Alkaline Phosphatase Sucrase-Isomaltase Complex Aminopeptidases CD13 Antigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Osborn M
Max Planck Institute for Biophysical Chemistry, Goettingen, Federal Republic of Germany.
Mazzoleni G
Santini D
Marrano D
Martinelli G
Weber K
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Article Info
Journal
Virchows Archiv. A, Pathological anatomy and histopathology
Abbr.
Virchows Arch A Pathol Anat Histopathol
ISSN
0174-7398
DOI
10.1007/BF00783022
Published
1988-00-00
Pages
303-12
Language
English
Region
Germany
NLM ID
8302198
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00783022
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